Impedimetric or Ion-Sensitive Field-Effect Transistor (ISFET) Aptasensors Based on the Self-Assembly of Au Nanoparticle-Functionalized Supramolecular Aptamer Nanostructures

Two electrical methods (Faradaic impedance spectroscopy and ion‐sensitive field‐effect transistor (ISFET)) are applied to sense aptamer‐substrate complexes. The methods utilize anticocaine aptamer fragments that self‐assemble, in the presence of cocaine, to a supramolecular aptamer fragments/cocaine...

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Bibliographic Details
Published in:Electroanalysis (New York, N.Y.) N.Y.), 2009-06, Vol.21 (11), p.1291-1296
Main Authors: Sharon, Etery, Freeman, Ronit, Tel-Vered, Ran, Willner, Itamar
Format: Article
Language:English
Subjects:
Aptamers
Cocaine
Field-effect-Transistors
Impedance
Sensors
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Summary:Two electrical methods (Faradaic impedance spectroscopy and ion‐sensitive field‐effect transistor (ISFET)) are applied to sense aptamer‐substrate complexes. The methods utilize anticocaine aptamer fragments that self‐assemble, in the presence of cocaine, to a supramolecular aptamer fragments/cocaine complex on the electrode surface or ISFET gate. One of the aptamer fragments is assembled on a Au electrode or the ISFET gate. The second thiolated aptamer fragment is used to modify Au NPs that are used as amplifying labels for the two detection schemes. The impedimetric and ISFET methods enabled the analysis of cocaine with detection limits of 1×10−5 M and 1×10−6 M, respectively.
ISSN:1040-0397
1521-4109
DOI:10.1002/elan.200804565