Loading…

Microsphere-based tracing and molecular delivery in embryonic stem cells

Abstract Embryonic stem (ES) cells are in vitro cell lines that can differentiate into all lineages of the fetus and the adult. Despite the versatility of genetic manipulation in murine ES cells, these approaches are time-consuming and rely on inefficient transient cellular delivery systems that can...

Full description

Saved in:
Bibliographic Details
Published in:Biomaterials 2009-10, Vol.30 (29), p.5853-5861
Main Authors: Tsakiridis, Anestis, Alexander, Lois M, Gennet, Nicole, Sanchez-Martin, Rosario M, Livigni, Alessandra, Li, Meng, Bradley, Mark, Brickman, Joshua M
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract Embryonic stem (ES) cells are in vitro cell lines that can differentiate into all lineages of the fetus and the adult. Despite the versatility of genetic manipulation in murine ES cells, these approaches are time-consuming and rely on inefficient transient cellular delivery systems that can only be applied to undifferentiated ES cell cultures. Here we describe a polystyrene microsphere-based system designed to efficiently deliver biological materials into both undifferentiated and differentiating ES cells. Our results demonstrate that these microspheres can be successfully employed for simultaneous cellular labeling and controlled transfer of various cargos such as fluorophores, proteins and nucleic acids into ES cells without any significant toxicity or loss of pluripotency. This versatile delivery system is also effective in other stem cell lines derived from early embryos, trophoblast and neural stem cells.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2009.06.024