Enrichment of a K-strategist microbial population able to biodegrade p-nitrophenol in a sequencing batch reactor

• Published in: Water research (Oxford) 2009-08, Vol.43 (15), p.3871-3883
• Main Authors: Martín-Hernández, Mariángel, Carrera, Julián, Pérez, Julio, Suárez-Ojeda, María Eugenia
• Format: Article
• Language: English
• Subjects: Activated sludge; aerobic conditions; Applied sciences; Bacteria - metabolism; biodegradation; Biodegradation, Environmental; Biological treatment; Biomass; bioreactors; Bioreactors - microbiology; chemical oxygen demand; Enrichment; Exact sciences and technology; Haldane kinetics; inoculum; K-strategist; kinetics; Mathematical models; Microorganisms; Modelling; Models, Biological; Nitrophenols - metabolism; optimization; Other industrial wastes. Sewage sludge; oxygen; Oxygen uptake rate; p-Nitrophenol; Pollution; Reactors; Sequencing; Strategy; Waste water; Wastes; wastewater treatment; Water Pollutants, Chemical - metabolism; Water Purification - instrumentation; Water Purification - methods; Water treatment and pollution
• ISSN: 0043-1354; 1879-2448
• DOI: 10.1016/j.watres.2009.06.001

The biological treatment of a high-strength p-nitrophenol (PNP) wastewater in an aerobic Sequencing Batch Reactor (SBR) has been studied. A specific operational strategy was applied with the main aim of developing a K-strategist PNP-degrading activated sludge. The enrichment of a K-strategist microbial population was performed using a non-acclimated biomass coming from a municipal WWTP as inoculum, and following a feeding strategy in which the PNP-degrading biomass was under endogenous conditions during more than 50% of the aerobic reaction phase. Hundred per cent of PNP removal was achieved in the whole operating period with a maximum specific PNP loading rate of 0.26 g PNP g −1 VSS d −1. A kinetic characterization of the obtained PNP-degrading population was carried out using respirometry assays in specifically designed batch tests. With the experimental data obtained a kinetic model including substrate inhibition has been used to describe the time-course of the PNP concentration and specific oxygen uptake rate (SOUR), simultaneously. The kinetic parameters obtained through optimization, validated with an additional respirometric test, were k max = 1.02 mg PNP mg −1 COD d −1, K s = 1.6 mg PNP L −1 and K i = 54 mg PNP L −1. The values obtained for the K s and k max are lower than those reported in the literature for mixed populations, meaning that the biomass is a K-strategist type, and therefore demonstrating the success of the operational strategy imposed to obtain such a K-strategist population. Moreover, our measured K i value is higher than those reported by most of the bibliographic references; therefore the acclimated activated sludge used in this work was evidently more adapted to PNP inhibition than the other reported cultures.