Proteomic profiling of snake venom metalloproteinases (SVMPs): Insights into venom induced pathology

Bothrops sp. snakebites account for the majority of envenomations in South and Central America. Bothrops jararaca accidents are characterized by edema, hemorrhage and necrosis, mainly attributed to the action of hemorrhagic snake venom metalloproteinases (SVMPs). Interestingly, accidents involving B...

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Bibliographic Details
Published in:Toxicon (Oxford) 2009-11, Vol.54 (6), p.836-844
Main Authors: Terra, Renata M.S., Pinto, Antônio F.M., Guimarães, Jorge A., Fox, Jay W.
Format: Article
Language:English
Subjects:
Animal poisons toxicology. Antivenoms
Animals
Biological and medical sciences
Bothrops
Bothrops jararaca
Bothrops lanceolatus
Chromatography, Liquid
Electrophoresis, Polyacrylamide Gel
Hydrolysis
Medical sciences
Metalloproteases
Metalloproteases - metabolism
Proteome
Snake venom
Snake Venoms - enzymology
Snake Venoms - toxicity
SVMP
Tandem Mass Spectrometry
Toxicology
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Summary:Bothrops sp. snakebites account for the majority of envenomations in South and Central America. Bothrops jararaca accidents are characterized by edema, hemorrhage and necrosis, mainly attributed to the action of hemorrhagic snake venom metalloproteinases (SVMPs). Interestingly, accidents involving Bothrops lanceolatus (Fer-de-Lance) have a prothrombotic profile with necrosis and hemorrhage rarely reported. Here we describe biochemical and proteomic approaches to compare the venom composition of these snakes, focusing on the presence and activity of SVMPs. The total relative amount of SVMPs was found to be approximately the same in the venom of both species, the difference being in the distribution of SVMPs subgroups. Fer-de-Lance venom has relatively more PI SVMPs peptides identified (23–16%) while Jararaca venom has a higher amount of PIII SVMPs (54–43%). Gelatinolytic activity in the PIII mass range is also higher in Jararaca venom. Interestingly, the homologous band region in the Fer-de-Lance zymogram was only very weakly gelatinolytic. According to these findings it is feasible that the different distribution of SVMPs subgroups and their particular biochemical and pharmacological characteristics are two of the main factors contributing to these two radically different venom induced pathologies.
ISSN:0041-0101
1879-3150
DOI:10.1016/j.toxicon.2009.06.010