Cu(2+)-selective naked-eye and fluorescent probe: its crystal structure and application in bioimaging

A new fluorescent and colorimetric Cu(2+) probe was synthesized, and realized optical imaging in RAW264.7 macrophages. The design strategy was based on a change in structure between spirocyclic (non-fluorescent) and ring-open (fluorescent) forms of rhodamine-based dyes, and its crystal structure was...

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Bibliographic Details
Published in:Analyst (London) 2009-09, Vol.134 (9), p.1826-1833
Main Authors: Yu, F., Zhang, W, Li, P., Xing, Y, Tong, L, Ma, J., Tang, B
Format: Article
Language:English
Online Access:Get full text
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Summary:A new fluorescent and colorimetric Cu(2+) probe was synthesized, and realized optical imaging in RAW264.7 macrophages. The design strategy was based on a change in structure between spirocyclic (non-fluorescent) and ring-open (fluorescent) forms of rhodamine-based dyes, and its crystal structure was presented to explain the binding mode. Upon treatment with Cu(2+), the weakly fluorescent probe exhibited a strong fluorescence response, high selectivity and was quantitative for Cu(2+) under physiological conditions. In addition, the off-on-type fluorescent change upon the addition of Cu(2+) was also applied in bioimaging.
ISSN:0003-2654
DOI:10.1039/b823360h