Immobilization of acetylcholineesterase–choline oxidase on a gold–platinum bimetallic nanoparticles modified glassy carbon electrode for the sensitive detection of organophosphate pesticides, carbamates and nerve agents

• Published in: Biosensors & bioelectronics 2009-12, Vol.25 (4), p.832-838
• Main Authors: Upadhyay, Sanjay, Rao, Golime Rama, Sharma, Mukesh K., Bhattacharya, Bijoy K., Rao, Vepa K., Vijayaraghavan, R.
• Format: Article
• Language: English
• Subjects: Acetylcholineesterase; Acetylcholinesterase - chemistry; Alcohol Oxidoreductases - chemistry; Aldicarb; Au–PtNPs; Biological and medical sciences; Biosensing Techniques - instrumentation; Biotechnology; Carbamates - analysis; Carbon - chemistry; Choline oxidase; Electrochemistry - instrumentation; Electrodes; Enzymes, Immobilized - chemistry; Equipment Design; Equipment Failure Analysis; Fundamental and applied biological sciences. Psychology; General aspects; Gold - chemistry; Immobilization techniques; Methods. Procedures. Technologies; Nanoparticles - chemistry; Nanoparticles - ultrastructure; Neurotoxins - analysis; Organophosphates - analysis; Paraoxon ethyl; Pesticides - analysis; Platinum - chemistry; Sarin
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2009.08.036

A novel, highly sensitive amperometric biosensor, based on electrodeposition of gold–platinum bimetallic nanoparticles onto 3-aminopropyltriethoxy silane modified glassy carbon electrode for the detection of paraoxon ethyl, aldicarb, and sarin has been developed. The biosensor consists of acetylcholineesterase (AChE)/choline oxidase (ChOx) immobilized by cross-linking with glutaraldehyde on a modified electrode. The properties of nanoparticles modified electrodes are characterized by scanning electron microscopy (SEM), energy dispersive X-ray (EDX), cyclic voltammograms (CVs) and electrochemical impedance spectroscopy (EIS). The synergistic action of Au and Pt nanoparticles showed excellent electrocatalytic activity with low applied potential for the detection of hydrogen peroxide (H2O2). The IC50 and inhibition rate constant (Ki) values were determined for the inhibitors using immobilized enzymes on modified electrode and the data were compared by spectrophotometric determination of these kinetic parameters using free enzymes in solution. Paraoxon ethyl, sarin, and aldicarb could be detected up to 150–200nM, 40–50nM, and 40–60μM respectively at 30–40% inhibition level of AChE enzyme and followed linearity in wide range concentration.