Estimation of sugars in milk by HPLC and its application in detection of adulteration of milk with soymilk

A high-performance liquid chromatography method is described for the detection of adulteration of milk with soymilk, based on separation of sugars on NH₂ column and their detection by refractive index detector. Sugars were extracted with 20% acetonitrile in the presence of Carrez solutions and quant...

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Bibliographic Details
Published in:International journal of dairy technology 2009-11, Vol.62 (4), p.514-519
Main Authors: SHARMA, RAJAN, RAJPUT, YUDHISHTHIR S, POONAM, DOGRA, GAURAV, TOMAR, SUDHIR K
Format: Article
Language:English
Subjects:
adulterated products
Adulteration
Biological and medical sciences
Chromatography
Food industries
Fundamental and applied biological sciences. Psychology
General aspects
HPLC
Lactose
Methods of analysis, processing and quality control, regulation, standards
Milk
Milk and cheese industries. Ice creams
Plant-based beverages
Soy products
Soymilk
Stachyose
Studies
Sugars
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Summary:A high-performance liquid chromatography method is described for the detection of adulteration of milk with soymilk, based on separation of sugars on NH₂ column and their detection by refractive index detector. Sugars were extracted with 20% acetonitrile in the presence of Carrez solutions and quantified. Recovery of added raffinose to soymilk was 96.3%. Owing to relative high concentration of lactose in milk or adulterated milk, lactose peak was very broad and spread to retention time corresponding to sucrose and raffinose. However, stachyose peak remained well separated. Presence of stachyose peak in milk can be used for the detection of adulteration of milk with soymilk and the method can detect upto 5% soymilk in milk.
ISSN:1364-727X
1471-0307
DOI:10.1111/j.1471-0307.2009.00532.x