Effects of Alloxan-lnduced Diabetes on the Expression of Insulin Signal Transmission Molecules

The mRNA of insulin receptor (JR.), the insulin receptor substrates (IRSs), glucose transporter 2 (GLUT2) and glu-cokinase (GK) in alloxan-induced diabetic rat livers were amplified by RT-PCR. The protein of the insulin receptor in rat livers was determined by western-blotting. The results show that...

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Bibliographic Details
Published in:Wuhan University journal of natural sciences 2009-10, Vol.14 (5), p.447-451
Main Authors: Ge, Yili, Peng, Hong, Sheng, Xiqun, Zhang, Gongchen, Zhou, Jun, Wang, Miao, Zhou, Xiaoping, Huang, Kaixun
Format: Article
Language:English
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Summary:The mRNA of insulin receptor (JR.), the insulin receptor substrates (IRSs), glucose transporter 2 (GLUT2) and glu-cokinase (GK) in alloxan-induced diabetic rat livers were amplified by RT-PCR. The protein of the insulin receptor in rat livers was determined by western-blotting. The results show that IR expression level decreased at the level of mRNA and protein. The gene expressions of IRSs, GLUT2 and GK changed significantly. The hepatic glycogen content in alloxan-diabetic rats treated with insulin ((13.2 0.4) mg ;g(-1)) did not restore to normal level ((17.0 c 0.4) mg ; g(-1)) by means of anthrone-H(2)SO(4) methods. The results imply that insulin resistance is developed during inchoate phase of alloxan-induced diabetic rats.
ISSN:1007-1202
DOI:10.1007/sl1859-009-0515-z