Adsorptive immobilization of enzymatic active substances on alumina–silica foam coated by carbon nanofibers

• Published in: Carbon (New York) 2009-02, Vol.47 (2), p.420-427
• Main Authors: Kovalenko, G.A., Perminova, L.V., Chuenko, T.V., Rudina, N.A.
• Format: Article
• Language: English
• Subjects: Adsorption; Bacteria; Carbon; Ceramics; Chlorofluorocarbons; Enzymes; Glucoamylase; Yeast
• ISSN: 0008-6223; 1873-3891
• DOI: 10.1016/j.carbon.2008.10.019

The adsorption abilities of an alumina–silica foam coated by a layer of catalytic filamentous carbon (CFC/ceramics) were studied with respect to enzymatic active substances (EAS) - enzymes glucoamylase and invertase, intracellular compartments of baker’s yeast and whole non-growing cells of various microorganisms ( Saccharomyces cerevisiae, Rhodococcus sp., Arthrobacter sp.). The activity and stability of heterogeneous biocatalysts prepared by EAS adsorption on the CFC/ceramics were studied in hydrolysis, oxidation and isomerization reactions. Glucoamylase was found to retain up to 20% of the activity of a soluble enzyme after adsorption on the CFC/ceramics. Invertase and invertase-active yeast autolysate retained practically all their activity. The enzymatic activities of non-growing cells of baker’s yeast and Rhodococcus increased after adsorption on CFC/ceramics by a factor of 1.5–1.8 and 3.7, respectively. A “glucoamylase on CFC/ceramics” biocatalyst for the hydrolysis of dextrin was shown to have high long-term stability, retaining its activity after storage for 10–12 months at ambient temperature. A “yeast autolysate on CFC/ceramics” biocatalyst for sucrose inversion retained >50% of the activity after 2–3 month storage at ambient temperature. A “non-growing bacteria Arthrobacter sp. on CFC/ceramics” biocatalyst for glucose isomerization retained half its activity after 12 h use at 70 °C.