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Production of 3-hydroxypropionic acid from glycerol by a novel recombinant Escherichia coli BL21 strain
3-Hydroxypropionic acid (3-HP) is an important platform chemical from which several commodity and specialty chemicals can be generated. The present investigation focuses on the construction and evaluation of a recombinant strain Escherichia coli SH254 that produces 3-HP from glycerol. The strain was...
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Published in: | Process biochemistry (1991) 2008-12, Vol.43 (12), p.1440-1446 |
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Main Authors: | , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | 3-Hydroxypropionic acid (3-HP) is an important platform chemical from which several commodity and specialty chemicals can be generated. The present investigation focuses on the construction and evaluation of a recombinant strain
Escherichia coli SH254 that produces 3-HP from glycerol. The strain was developed by cloning two genes,
dhaB of
Klebsiella pneumoniae DSM 2026 encoding glycerol dehydratase and
aldH of
E. coli K-12 MG1655 encoding aldehyde dehydrogenase, respectively.
In vitro assays of crude enzyme extract of glycerol dehydratase (DhaB) showed 37.0
U
mg
−1 protein on glycerol with coenzyme B
12, and partially purified aldehyde dehydrogenase (AldH) exhibited 22.8
U
mg
−1 protein on 3-hydroxypropionaldehyde (3-HPA) with NAD
+ as a cofactor. When cultivated aerobically on a glycerol medium containing yeast extract, the recombinant
E. coli SH254 produced 3-HP at a maximum of 6.5
mmol
l
−1 (0.58
g
l
−1). The highest specific rate and yield of 3-HP production were estimated as 6.6
mmol
g
−1
cdw
h
−1 and 0.48
mol
mol
−1 glycerol, respectively. Although not optimized extensively, this study is encouraging for further development of a bioprocess to produce 3-HP from glycerol. |
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ISSN: | 1359-5113 1873-3298 |
DOI: | 10.1016/j.procbio.2008.04.027 |