Silver nanoparticles inhibit VEGF induced cell proliferation and migration in bovine retinal endothelial cells

Angiogenesis, the growth of new blood vessels from pre-existing vasculature is of physiological and pathological importance. We have investigated the anti-angiogenic potential of silver nanoparticles, produced by Bacillus licheniformis. Bovine retinal endothelial cells (BRECs) were treated with the...

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Bibliographic Details
Published in:Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2009-10, Vol.73 (1), p.51-57
Main Authors: Kalishwaralal, Kalimuthu, Banumathi, Elayappan, Pandian, SureshBabu Ram Kumar, Deepak, Venkataraman, Muniyandi, Jeyaraj, Eom, Soo Hyun, Gurunathan, Sangiliyandi
Format: Article
Language:English
Subjects:
Angiogenesis Inhibitors - toxicity
Animals
Apoptosis - drug effects
Bacillus - chemistry
Bacillus licheniformis
Blotting, Western
Caspase 3 - metabolism
Cattle
Cell Movement - drug effects
Cell Proliferation - drug effects
Cell Survival - drug effects
Cells, Cultured
Diabetic retinopathy
DNA Fragmentation - drug effects
Dose-Response Relationship, Drug
Endothelial Cells - cytology
Endothelial Cells - drug effects
Endothelial Cells - metabolism
Inhibitory Concentration 50
Metal Nanoparticles - chemistry
Metal Nanoparticles - toxicity
Neovascularization, Physiologic - drug effects
Phosphorylation - drug effects
Primary bovine retinal endothelial cells
Proto-Oncogene Proteins c-akt - metabolism
Retina - cytology
Silver - chemistry
Silver nanoparticles
Vascular Endothelial Growth Factor A - pharmacology
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Summary:Angiogenesis, the growth of new blood vessels from pre-existing vasculature is of physiological and pathological importance. We have investigated the anti-angiogenic potential of silver nanoparticles, produced by Bacillus licheniformis. Bovine retinal endothelial cells (BRECs) were treated with the different concentrations of silver nanoparticles for 24 h in the presence and absence of vascular endothelial growth factor (VEGF), where 500 nM (IC 50) of silver nanoparticle concentration, was able to block the proliferation and migration of BRECs. The cells showed a clear enhancement in caspase-3 activity and formation of DNA ladders, evidence of induction of apoptosis. Here we report for the first time that silver nanoparticles inhibit cell survival via PI3K/Akt dependent pathway in Bovine retinal endothelial cells.
ISSN:0927-7765
1873-4367
DOI:10.1016/j.colsurfb.2009.04.025