Microbial production of 2,3-butanediol by a mutagenized strain of Serratia marcescens H30

The production of 2,3-butanediol (2,3-BD) by Serratia marcescens H30 from sucrose was studied. Medium composition for 2,3-BD production by S. marcescens H30 was optimized in shake flask fermentations using Plackett–Burman design (PB) and response surface methodology (RSM). Results indicated that yea...

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Bibliographic Details
Published in:Bioresource technology 2010-03, Vol.101 (6), p.1961-1967
Main Authors: Zhang, Liaoyuan, Yang, Yunlong, Sun, Jian’an, Shen, Yaling, Wei, Dongzhi, Zhu, Jiawen, Chu, Ju
Format: Article
Language:English
Subjects:
2,3-Butanediol
Biological and medical sciences
Butylene Glycols - chemistry
Butylene Glycols - metabolism
Chromatography, Gel
Chromatography, Ion Exchange - methods
Electrophoresis, Polyacrylamide Gel
Fed-batch
Fibrinogen - chemistry
Fundamental and applied biological sciences. Psychology
Hydrogen-Ion Concentration
Isoelectric Focusing
Medium optimization
Molecular Weight
Mutagenesis
Peptide Hydrolases - chemistry
Respiratory quotient
Serine Proteases - chemistry
Serratia marcescens
Serratia marcescens - metabolism
Serratia marcescens H30
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Temperature
Thrombosis - metabolism
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Summary:The production of 2,3-butanediol (2,3-BD) by Serratia marcescens H30 from sucrose was studied. Medium composition for 2,3-BD production by S. marcescens H30 was optimized in shake flask fermentations using Plackett–Burman design (PB) and response surface methodology (RSM). Results indicated that yeast extract and sodium acetate had significant effects on the 2,3-BD production. And their optimal concentrations were determined by RSM. The optimal medium was used to perform fermentation experiments by S. marcescens H30 in a 3.7 l bioreactor. Several feeding strategies including interim feeding, exponential feeding and constant residual sucrose concentration feeding were compared for improving 2,3-BD production. Ultimately, a suitable control strategy which combined the respiratory quotient (RQ) control with the constant residual sucrose concentration fed-batch was developed. Using this strategy, the maximum 2,3-BD concentration of 139.92 g/l with the diol (AC + BD) productivity of 3.49 g/l h and the yield of 94.67% was obtained.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2009.10.052