Rapid detection of Salmonella in milk by electrochemical magneto-immunosensing

A very simple and rapid method for the detection of Salmonella in milk is reported. In this approach, the bacteria are captured and preconcentrated from milk samples with magnetic beads through an immunological reaction. A second polyclonal antibody labeled with peroxidase is used as serological con...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2009-10, Vol.25 (2), p.510-513
Main Authors: Liébana, Susana, Lermo, Anabel, Campoy, Susana, Cortés, María Pilar, Alegret, Salvador, Pividori, María Isabel
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Biosensing Techniques - instrumentation
Biosensors
Biotechnology
Cattle
Electrochemical immunosensor
Electrochemistry - instrumentation
Electrodes
Equipment Design
Equipment Failure Analysis
Escherichia coli
Food Analysis - instrumentation
Food Contamination - analysis
Fundamental and applied biological sciences. Psychology
Immunomagnetic separation
Immunomagnetic Separation - instrumentation
Magnetic bead
Magneto-electrode
Methods. Procedures. Technologies
Milk - microbiology
Salmonella
Salmonella - isolation & purification
Transducers
Various methods and equipments
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Summary:A very simple and rapid method for the detection of Salmonella in milk is reported. In this approach, the bacteria are captured and preconcentrated from milk samples with magnetic beads through an immunological reaction. A second polyclonal antibody labeled with peroxidase is used as serological confirmation with electrochemical detection based on a magneto-electrode. The ‘IMS/m-GEC electrochemical immunosensing’ approach shows a limit of detection of 5×103 and 7.5×103CFUmL−1 in LB and in milk diluted 1/10 in LB broth, respectively, in 50min without any pretreatment. If the skimmed-milk is preenriched for 6h, the method is able to detect as low as 1.4CFUmL−1, while if it is preenriched for 8h, as low as 0.108×CFUmL−1 (2.7×CFU in 25g of milk, in 5 samples of 5mL) are detected accordingly with the legislation. Moreover, the method is able to clearly distinguish between food pathogenic bacteria such as Salmonella and Escherichia coli. The features of this approach are discussed and compared with classical culture methods.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2009.07.022