efficacy and immunogenicity of a live transconjugant hybrid strain of Shigella dysenteriae type 1 in two animal models

In our earlier studies, we constructed a hybrid strain of Shigella dysenteriae type 1 by introducing a plasmid vector pPR 1347. After introduction of a lipopolysaccharide (LPS) biosynthesis gene, virulent Shigella dysenteriae type 1 strain became avirulent. In our present study, we have evaluated th...

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Bibliographic Details
Published in:World journal of microbiology & biotechnology 2009-04, Vol.25 (4), p.679-686
Main Authors: Koley, Hemanta, Barman, Soumik, Roy, Nivedita, Saha, Dhira Rani, Kumar, Ranajit
Format: Article
Language:English
Subjects:
Animal models
Animals
Applied Microbiology
Bacterial infections
Biochemistry
Biological and medical sciences
Biomedical and Life Sciences
Biosynthesis
Biotechnology
Cholera
Colon
Developing countries
Drug resistance
Environmental Engineering/Biotechnology
Epidemics
Fundamental and applied biological sciences. Psychology
Genes
Immune response
Immunization
Immunogenicity
Ketamine
LDCs
Life Sciences
Mathematical analysis
Mice
Microbiology
Original Paper
Rabbits
Salmonella
Sanitation
Serums
Shigella
Shigella dysenteriae
Shigellosis
Strain
Studies
Vaccines
Virulence
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Summary:In our earlier studies, we constructed a hybrid strain of Shigella dysenteriae type 1 by introducing a plasmid vector pPR 1347. After introduction of a lipopolysaccharide (LPS) biosynthesis gene, virulent Shigella dysenteriae type 1 strain became avirulent. In our present study, we have evaluated the immune response and protective efficacy of avirulent live transconjugant Shigella hybrid (LTSH) strain against wild type Shigella dysenteriae type 1, after four doses of oral (rabbit) and intranasal (mouse) immunizations. Serum IgG titers showed exponential increase during immunization and peaking on the 28th day and remained at that level till the 35th day in both the rabbit and the mouse models. When tested, serum IgG titers persisted for 63 days in mice and relatively high for 150 days in case of rabbits. Protection studies showed 100% protection against the challenge with wild type Shigella dysenteriae type 1 strain in rabbits and 80% protection in mice. Our results suggested that the LTSH strain could be a useful vaccine candidate strain in the future.
ISSN:0959-3993
1573-0972
DOI:10.1007/s11274-008-9937-6