Bioactive metabolites from Penicillium sp., an endophytic fungus residing in Hopea hainanensis

The metabolites of endophytic fungus Penicillium sp. from the leaf of Hopea hainanensis were reported for the first time. By bioassay-guided fractionation, the EtOAc extract of a solid-matrix steady culture of this fungus afforded six compounds, which were identified through a combination of spectra...

Full description

Saved in:
Bibliographic Details
Published in:World journal of microbiology & biotechnology 2008-10, Vol.24 (10), p.2143-2147
Main Authors: Wang, F. W, Hou, Z. M, Wang, C. R, Li, P, Shi, D. H
Format: Article
Language:English
Subjects:
Acids
Applied Microbiology
Aspergillus niger
Bioassays
Biochemistry
Biological and medical sciences
Biomedical and Life Sciences
Biotechnology
Candida albicans
Chromatography
Cytotoxicity
Environmental Engineering/Biotechnology
Fractionation
Fundamental and applied biological sciences. Psychology
Fungi
Leaves
Life Sciences
Metabolites
Microbiology
Original Paper
Penicillium
Studies
Trichophyton rubrum
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The metabolites of endophytic fungus Penicillium sp. from the leaf of Hopea hainanensis were reported for the first time. By bioassay-guided fractionation, the EtOAc extract of a solid-matrix steady culture of this fungus afforded six compounds, which were identified through a combination of spectral and chemical methods (IR, MS, ¹H- and ¹³C-NMR) to be monomethylsulochrin (1), rhizoctonic acid (2), asperfumoid (3), physcion (4), 7,8-dimethyl-iso-alloxazine (5) and 3,5-dichloro-p-anisic acid (6). Compounds 2, 3 and 6 were obtained from Penicillium sp. for the first time. All of the six isolates were subjected to in vitro bioactive assays including antifungal action against three human pathogenic fungi Candida albicans, Trichophyton rubrum and Aspergillus niger and cytotoxic activity against the human nasopharyngeal epidermoid tumor KB cell line and human liver cancer HepG2 cell line. As a result, compounds 2-4 and 6 inhibited the growth of C. albicans with MICs of 40.0, 20.0, 50.0 and 15.0 μg/ml, respectively and the compound 6 showed growth inhibition against A. niger with MICs of 40.0 μg/ml. In addition, compounds 1-3 and 6 exhibited cytotoxic activity against KB cell line with IC₅₀ value of 30.0, 20.0, 20.0, 5.0 μg/ml, respectively and against HepG2 cell line with IC₅₀ value of 30.0, 25.0, 15.0, 10.0 μg/ml, respectively.
ISSN:0959-3993
1573-0972
DOI:10.1007/s11274-008-9720-8