Genotoxicity monitoring of freshwater environments using caged carp (Cyprinus carpio)

The present study deals with genotoxicity assessment of freshwaters using caged carp (Cyprinus carpio). Carps were transplanted from a fish-farm to three differently polluted sites in eastern Croatia. Two polluted sites were situated in the river Drava, downstream from the cities of Belišće and Osij...

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Bibliographic Details
Published in:Ecotoxicology (London) 2010, Vol.19 (1), p.77-84
Main Authors: Klobučar, Göran I. V, Štambuk, Anamaria, Pavlica, Mirjana, Sertić Perić, Mirela, Kutuzović Hackenberger, Branimir, Hylland, Ketil
Format: Article
Language:English
Subjects:
Animals
Anthropogenic factors
Aquaria
Aquariums
Aquatic ecosystems
Assaying
Bioassays
Carp
Carps - metabolism
Comet Assay
Comets
Croatia
Cyprinus carpio
Damage
Deoxyribonucleic acid
DNA
DNA Damage - drug effects
Earth and Environmental Science
Ecology
Ecotoxicology
Environment
Environmental Management
Environmental Monitoring - methods
Fresh water
Freshwater
Freshwater ecosystems
Freshwater environments
Freshwaters
Genotoxicity
Micronucleus Tests
Pollution
Protection and preservation
Rivers
Sensitivity and Specificity
Statistics, Nonparametric
Water Pollutants, Chemical - toxicity
Water pollution
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Summary:The present study deals with genotoxicity assessment of freshwaters using caged carp (Cyprinus carpio). Carps were transplanted from a fish-farm to three differently polluted sites in eastern Croatia. Two polluted sites were situated in the river Drava, downstream from the cities of Belišće and Osijek, while the reference site was in the Nature Park Kopački rit, a preserved wetland area with limited anthropogenic influence. Exposure lasted for 3 weeks and was repeated for 3 years (2002-2004). DNA damage was assessed in erythrocytes of the exposed animals by the Comet assay and micronucleus test (MNT). In order to evaluate possible differences in stress responses to polluted water in situ and in aquaria a laboratory exposure was performed with water from the studied location in the second year of the study. Carp from the sites with high anthropogenic influence (Belišće and Osijek) had higher average DNA damage as expressed in both the MNT and Comet assay. Of the two, the Comet assay appeared to be more sensitive following both caging and aquaria exposures. The results from this study suggest that 3 weeks caging exposure of C. carpio may be a useful strategy to monitor for genotoxic agents in freshwater ecosystems.
ISSN:0963-9292
1573-3017
DOI:10.1007/s10646-009-0390-6