Kinetic Characterization by Surface Plasmon Resonance-Based Biosensors: Principle and Emerging Trends

Within the last three decades, surface plasmon resonance (SPR)-based optical biosensors have emerged as leading instruments for the study of biomolecular interactions. The rapid dissemination of the technology has provided researchers with versatile and sensitive instruments for the identification o...

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Bibliographic Details
Published in:Cellular and molecular bioengineering 2008-12, Vol.1 (4), p.204-215
Main Authors: De Crescenzo, Gregory, Boucher, Cyril, Durocher, Yves, Jolicoeur, Mario
Format: Article
Language:English
Subjects:
Bioengineering
Biological and Medical Physics
Biomaterials
Biomedical Engineering and Bioengineering
Biomedical Engineering/Biotechnology
Biophysics
Biosensors
Cell Biology
Data collection
Engineering
Kinetics
Molecular biology
Proteins
Resonance
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Summary:Within the last three decades, surface plasmon resonance (SPR)-based optical biosensors have emerged as leading instruments for the study of biomolecular interactions. The rapid dissemination of the technology has provided researchers with versatile and sensitive instruments for the identification of kinetic and thermodynamic parameters related to macromolecular interactions. This review article presents the basic principles, as well as the classical experimental approaches and data treatment techniques related to the use of commercially available instruments for the characterization of protein–protein interactions. Emphasis will be put on new experimental trends aiming at improving two important aspects of any SPR kinetic studies, i.e., proteins tethering on biosensor surfaces as well as the optimization of data collection.
ISSN:1865-5025
1865-5033
DOI:10.1007/s12195-008-0035-5