Production of copolymer poly(3-hydroxybutyrate-co-4-hydroxybutyrate) through a one-step cultivation process

A one-step cultivation process for the production of biodegradable polymer poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] by Cupriavidus sp. USMAA2-4 was carried out using various carbon sources. It was found that Cupriavidus sp. USMAA2-4 could produce approximately 44 wt.% copolymer o...

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Bibliographic Details
Published in:World journal of microbiology & biotechnology 2008-11, Vol.24 (11), p.2403-2409
Main Authors: Rahayu, A, Zaleha, Z, Yahya, Ahmad R. M, Majid, M. I. A, Amirul, A. A
Format: Article
Language:English
Subjects:
Acids
Applied Microbiology
Biochemistry
Biodegradable materials
Biodegradation
Biological and medical sciences
Biomedical and Life Sciences
Biotechnology
Carbon
Carbon sources
Copolymers
Cultivation
Environmental Engineering/Biotechnology
Fundamental and applied biological sciences. Psychology
Life Sciences
Microbiology
Microorganisms
Molecular weight
Nitrogen
NMR
Nuclear magnetic resonance
Original Paper
Plastics
Polyhydroxyalkanoates
Polymers
Studies
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Summary:A one-step cultivation process for the production of biodegradable polymer poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] by Cupriavidus sp. USMAA2-4 was carried out using various carbon sources. It was found that Cupriavidus sp. USMAA2-4 could produce approximately 44 wt.% copolymer of P(3HB-co-4HB) with 27 mol% 4HB composition when the combination of oleic acid and 1,4-butanediol are used as carbon sources in 60 h cultivation. The manipulation of carbon-to-nitrogen ratio (C/N) resulted in the increase of dry cell weight, PHA content as well as 4HB composition. A new strategy of introducing oleic acid and 1,4-butanediol together and separately at different concentration demonstrated different yield in PHA content ranging from 47 to 58 wt.%. The molecular weight obtained was 234 kDa (by adding 1,4-butanediol and oleic acid together) and 212 kDa (by adding 1,4-butanediol separately). The copolymer of P(3HB-co-4HB) produced by Cupriavidus sp. USMAA2-4 was detected statistically as a random copolymer when analysed by nuclear magnetic resonance (NMR) spectroscopy.
ISSN:0959-3993
1573-0972
DOI:10.1007/s11274-008-9764-9