morphological and physiological evolution of Aspergillus terreus mycelium in the submerged culture and its relation to the formation of secondary metabolites

The influence of the morphology and differentiation of Aspergillus terreus hyphae on the formation of mevinolinic acid (lovastatin) and (+)-geodin was tested. Lovastatin titre was the highest (above 60 mg l⁻¹) in the system with smaller pellets (diameter below 1.5 mm) and high biomass concentration...

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Published in:World journal of microbiology & biotechnology 2010, Vol.26 (1), p.41-54
Main Authors: Bizukojc, M, Ledakowicz, S
Format: Article
Language:English
Subjects:
Applied Microbiology
Aspergillus terreus
Bacteria
Batch processes
Biochemistry
Biological and medical sciences
Biomedical and Life Sciences
Biosynthesis
Biotechnology
Drugs
Environmental Engineering/Biotechnology
Evolution
Experiments
Fundamental and applied biological sciences. Psychology
Fungi
Investigations
Life Sciences
Metabolites
Microbiology
Morphology
Original Paper
Penicillin
Pharmaceutical industry
Physiology
Secondary metabolites
Studies
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description The influence of the morphology and differentiation of Aspergillus terreus hyphae on the formation of mevinolinic acid (lovastatin) and (+)-geodin was tested. Lovastatin titre was the highest (above 60 mg l⁻¹) in the system with smaller pellets (diameter below 1.5 mm) and high biomass concentration (above 10 g l⁻¹ in the idiophase). These biomass features were induced by the higher initial number of spores in the preculture (above 2 × 10¹⁰ l⁻¹). At the initial number of spores below 2 × 10⁹ l⁻¹ (+)-geodin biosynthesis was the most efficient but it was rather connected with the elevated C/N ratio than with the pellet size. In order to quantify the hyphal differentiation in fungal pellets a special approach was used. The sectioning of the stained pellets together with the image analysis and calculation procedures were applied. The analysis of hyphal differentiation indicated that lovastatin formation was correlated with the fraction of the active, growing hyphae.
doi_str_mv 10.1007/s11274-009-0140-1
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source ABI/INFORM Global; Springer Nature
subjects Applied Microbiology
Aspergillus terreus
Bacteria
Batch processes
Biochemistry
Biological and medical sciences
Biomedical and Life Sciences
Biosynthesis
Biotechnology
Drugs
Environmental Engineering/Biotechnology
Evolution
Experiments
Fundamental and applied biological sciences. Psychology
Fungi
Investigations
Life Sciences
Metabolites
Microbiology
Morphology
Original Paper
Penicillin
Pharmaceutical industry
Physiology
Secondary metabolites
Studies
title morphological and physiological evolution of Aspergillus terreus mycelium in the submerged culture and its relation to the formation of secondary metabolites
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