Bovine papillomavirus infection in equine sarcoids and in bovine bladder cancers

Bovine papillomavirus (BPV) type 2 is involved in carcinogenesis of the urinary bladder in cattle, while BPV-1 is commonly associated with equine sarcoid tumours. In both cases the early viral proteins are expressed, but virion is not produced. Given the similarities in BPV biology between the tumou...

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Bibliographic Details
Published in:The veterinary journal (1997) 2007-11, Vol.174 (3), p.599-604
Main Authors: Yuan, Zhengqiang, Gallagher, Alice, Gault, Elizabeth A., Campo, M. Saveria, Nasir, Lubna
Format: Article
Language:English
Subjects:
Animals
bladder
bladder diseases
Bovine bladder cancers
Bovine papillomavirus
Bovine papillomavirus 1
Bovine papillomavirus 1 - isolation & purification
carcinogenesis
Cattle
cattle diseases
Cattle Diseases - virology
DNA
DNA, Viral
DNA, Viral - isolation & purification
Equine sarcoids
genes
genetic techniques and protocols
Horse Diseases
Horse Diseases - virology
Horses
isolation & purification
microbial genetics
Papillomaviridae
Plasmids
sarcoid
Skin Neoplasms
Skin Neoplasms - veterinary
Skin Neoplasms - virology
Urinary Bladder Neoplasms
Urinary Bladder Neoplasms - veterinary
Urinary Bladder Neoplasms - virology
veterinary
virology
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Summary:Bovine papillomavirus (BPV) type 2 is involved in carcinogenesis of the urinary bladder in cattle, while BPV-1 is commonly associated with equine sarcoid tumours. In both cases the early viral proteins are expressed, but virion is not produced. Given the similarities in BPV biology between the tumours in cattle and horses, bovine bladder cancers and equine sarcoids were compared with respect to physical status, load of viral DNA and variability of the E5 open reading frame (ORF). Rolling circle amplification demonstrated that BPV-1 and BPV-2 genomes exist as double stranded, episomal, circular forms in the two tumours. Realtime quantitative PCR revealed that equine sarcoids contained higher viral DNA loads compared to bovine bladder cancers. The BPV-1 E5 ORF showed sequence variation but BPV-2 ORF did not. The presence of BPV-1 E5 variations or their absence in the BPV-2 E5 ORF does not appear to have an effect on viral DNA load in either tumour type.
ISSN:1090-0233
1532-2971
DOI:10.1016/j.tvjl.2006.10.012