Fenugreek (Trigonella foenum graecum) Seed Extract Prevents Ethanol-Induced Toxicity and Apoptosis in Chang Liver Cells

The protective effect of a polyphenolic extract of fenugreek seeds (FPEt) against ethanol (EtOH)-induced toxicity was investigated in human Chang liver cells. Cells were incubated with either 30 mM EtOH alone or together in the presence of seed extract for 24 h. Assays were performed in treated cell...

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Bibliographic Details
Published in:Alcohol and alcoholism (Oxford) 2006-05, Vol.41 (3), p.267-273
Main Authors: Kaviarasan, Subramanian, Ramamurty, Nalini, Gunasekaran, Palani, Varalakshmi, Elango, Anuradha, Carani Venkatraman
Format: Article
Language:English
Subjects:
Alcohol consumption
Ethyl alcohol
Herbs
Liver diseases
Protection
Online Access:Get full text
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Summary:The protective effect of a polyphenolic extract of fenugreek seeds (FPEt) against ethanol (EtOH)-induced toxicity was investigated in human Chang liver cells. Cells were incubated with either 30 mM EtOH alone or together in the presence of seed extract for 24 h. Assays were performed in treated cells to evaluate the ability of seeds to prevent the toxic effects of EtOH. EtOH treatment suppressed the growth of Chang liver cells & induced cytotoxicity, oxygen radical formation & mitochondrial dysfunction. Reduced glutathione (GSH) concentration was decreased significantly (P < 0.05) while oxidized glutathione (GSSG) concentration was significantly elevated in EtOH-treated cells as compared with normal cells. Incubation of FPEt along with EtOH significantly increased cell viability in a dose-dependent manner, caused a reduction in lactate dehydrogenase leakage & normalized GSH/GSSG ratio. The extract dose-dependently reduced thiobarbituric acid reactive substances formation. Apoptosis was observed in EtOH-treated cells while FPEt reduced apoptosis by decreasing the accumulation of sub-G1 phase cells. The cytoprotective effects of FPEt were comparable with those of a positive control silymarin, a known hepatoprotective agent. The findings suggest that the polyphenolic compounds of fenugreek seeds can be considered cytoprotective during EtOH-induced liver damage. 2 Tables, 6 Figures, 34 References. Adapted from the source document.
ISSN:0735-0414
DOI:10.1093/alcalc/ag1020