Structural analysis and identification of cis-elements of rice osRACD gene

The osRACD gene correlated with fertility transformation in the photoperiod sensitive genic male sterile rice (PGMR), Nongken 58S, encoded a rice (Oryza sativa L. ssp. japonica) small GTPase belonging to the Rac/Rho family. Inverse PCR was performed to amplify a fragment about 1.4 kb in 5' upst...

Full description

Saved in:
Bibliographic Details
Published in:Acta biochimica et biophysica Sinica 2004-03, Vol.36 (3), p.191-198
Main Authors: Xu, Yun-Zhe, You, Rui-Lin, Wu, Nai-Hu
Format: Article
Language:English
Subjects:
5' Untranslated Regions - genetics
Base Sequence
Fertility - genetics
GTP-Binding Proteins - chemistry
GTP-Binding Proteins - genetics
GTP-Binding Proteins - metabolism
Molecular Sequence Data
Oryza - chemistry
Oryza - genetics
Oryza - metabolism
Photoperiod
Plant Proteins - chemistry
Plant Proteins - genetics
Plant Proteins - metabolism
Promoter Regions, Genetic - genetics
Regulatory Sequences, Nucleic Acid
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The osRACD gene correlated with fertility transformation in the photoperiod sensitive genic male sterile rice (PGMR), Nongken 58S, encoded a rice (Oryza sativa L. ssp. japonica) small GTPase belonging to the Rac/Rho family. Inverse PCR was performed to amplify a fragment about 1.4 kb in 5' upstream region of the osRACD promoter. Deletion mutation and gel mobility shift assay characterized two fragments (-799 to -686 nt, and -686 to -431 nt) in the osRACD promoter that could be involved in its transcriptional regulation. When these two deletion fragments were used as probe respectively, a retarded band appeared in the nuclear extracts of fertile 58S rice under short day (58S-SD). Whereas no retarded band was shown in the nuclear extracts of sterile 58S rice under long day (58S-LD). Competition assay indicated that the factors in the retarded bands binding to these two fragments were the same trans-acting factor (termed rice factor, RF). The binding affinity of RF was affected by phosphorylation and was higher in SD-growth rice than that of LD-growth rice.
ISSN:1672-9145
DOI:10.1093/abbs/36.3.191