Expression, crystallization and preliminary X-ray characterization of the Drosophila transcription factor Doublesex

Doublesex (DSX) is a transcription factor in Drosophila melanogaster that regulates somatic sexual differentiation. Homologues have been found in diverse metazoans, suggesting a conserved role in development. Here, the expression, purification and crystallization of a novel 63‐amino‐acid dimerizatio...

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Bibliographic Details
Published in:Acta crystallographica. Section D, Biological crystallography. Biological crystallography., 2004-07, Vol.60 (7), p.1328-1330
Main Authors: Bayrer, James, Wan, Zhuli, Li, Biaoru, Weiss, Michael A.
Format: Article
Language:English
Subjects:
Animals
Crystallization
Crystallography, X-Ray
dimerization
DM domain
DNA-Binding Proteins - chemistry
DNA-Binding Proteins - genetics
DNA-Binding Proteins - isolation & purification
Doublesex
Drosophila melanogaster - chemistry
Drosophila melanogaster - genetics
Drosophila Proteins - chemistry
Drosophila Proteins - genetics
Drosophila Proteins - isolation & purification
Gene Expression
sex determination
sex reversal
transcription factor
Transcription Factors - chemistry
Transcription Factors - genetics
Transcription Factors - isolation & purification
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Summary:Doublesex (DSX) is a transcription factor in Drosophila melanogaster that regulates somatic sexual differentiation. Homologues have been found in diverse metazoans, suggesting a conserved role in development. Here, the expression, purification and crystallization of a novel 63‐amino‐acid dimerization domain and selenomethionine analogue are described. Native crystals belong to space group P212121 and diffract to a resolution of 1.6 Å with synchrotron radiation. Selenomethionine‐containing crystals provide anomalous dispersion for phasing.
ISSN:1399-0047
0907-4449
1399-0047
DOI:10.1107/S0907444904012223