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Expression, crystallization and preliminary X-ray characterization of the Drosophila transcription factor Doublesex
Doublesex (DSX) is a transcription factor in Drosophila melanogaster that regulates somatic sexual differentiation. Homologues have been found in diverse metazoans, suggesting a conserved role in development. Here, the expression, purification and crystallization of a novel 63‐amino‐acid dimerizatio...
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| Published in: | Acta crystallographica. Section D, Biological crystallography. Biological crystallography., 2004-07, Vol.60 (7), p.1328-1330 |
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| container_end_page | 1330 |
| container_issue | 7 |
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| container_title | Acta crystallographica. Section D, Biological crystallography. |
| container_volume | 60 |
| creator | Bayrer, James Wan, Zhuli Li, Biaoru Weiss, Michael A. |
| description | Doublesex (DSX) is a transcription factor in Drosophila melanogaster that regulates somatic sexual differentiation. Homologues have been found in diverse metazoans, suggesting a conserved role in development. Here, the expression, purification and crystallization of a novel 63‐amino‐acid dimerization domain and selenomethionine analogue are described. Native crystals belong to space group P212121 and diffract to a resolution of 1.6 Å with synchrotron radiation. Selenomethionine‐containing crystals provide anomalous dispersion for phasing. |
| doi_str_mv | 10.1107/S0907444904012223 |
| format | article |
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| identifier | ISSN: 1399-0047 |
| ispartof | Acta crystallographica. Section D, Biological crystallography., 2004-07, Vol.60 (7), p.1328-1330 |
| issn | 1399-0047 0907-4449 1399-0047 |
| language | eng |
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| source | Wiley; Alma/SFX Local Collection |
| subjects | Animals Crystallization Crystallography, X-Ray dimerization DM domain DNA-Binding Proteins - chemistry DNA-Binding Proteins - genetics DNA-Binding Proteins - isolation & purification Doublesex Drosophila melanogaster - chemistry Drosophila melanogaster - genetics Drosophila Proteins - chemistry Drosophila Proteins - genetics Drosophila Proteins - isolation & purification Gene Expression sex determination sex reversal transcription factor Transcription Factors - chemistry Transcription Factors - genetics Transcription Factors - isolation & purification |
| title | Expression, crystallization and preliminary X-ray characterization of the Drosophila transcription factor Doublesex |
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