IgE testing in capillary blood

Serologic IgE testing is generally performed using serum, obtained by venepuncture. We tested whether paper‐absorbed and eluted capillary blood, obtained by a less invasive method (finger prick) could be used for allergy testing in young children. This was performed by comparative IgE testing, using...

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Bibliographic Details
Published in:Pediatric allergy and immunology 2004-06, Vol.15 (3), p.230-233
Main Authors: Stapel, Steven O., Eysink, Petra E. D., de Vrieze, Janny, Aalberse, Rob C.
Format: Article
Language:English
Subjects:
allergy
Biological and medical sciences
Blood Specimen Collection - methods
blood spot
Capillaries
Child, Preschool
Cough - blood
Cough - etiology
Cough - immunology
Fundamental and applied biological sciences. Psychology
Fundamental immunology
General aspects
Humans
immunoglobulin E
Immunoglobulin E - blood
Infant
Medical sciences
Radioallergosorbent Test
Respiratory Hypersensitivity - blood
Respiratory Hypersensitivity - diagnosis
Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis
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Summary:Serologic IgE testing is generally performed using serum, obtained by venepuncture. We tested whether paper‐absorbed and eluted capillary blood, obtained by a less invasive method (finger prick) could be used for allergy testing in young children. This was performed by comparative IgE testing, using paper‐absorbed blood/serum and serum. Practical applicability of the procedure was tested by assaying paper‐absorbed and eluted blood, obtained from 640 children with complaints of prolonged coughing, for IgE to airborne allergens. We found that IgE testing, using paper‐absorbed/eluted material and serum yields virtually identical results (mean ratio for positive samples: 1.01, 95% confidence interval: 0.58–1.75). Blood spot testing revealed that sensitization to inhalant allergens is not uncommon in preschool children (13% positive radioallergosorbent test [RAST] tests), which means that this procedure is a useful method for assaying allergic sensitization in children.
ISSN:0905-6157
1399-3038
DOI:10.1111/j.1399-3038.2004.00142.x