Structure of potato calmodulin PCM6: the first report of the three-dimensional structure of a plant calmodulin

The crystal structure of a potato calmodulin (PCM6) was solved by molecular replacement and refined to a crystallographic R factor of 22.8% (Rfree = 25.0%) using X‐­ray diffraction data in the resolution range 8.0–2.0 Å. This is the first report of the three‐dimensional structure of a plant Ca2+‐cal...

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Bibliographic Details
Published in:Acta crystallographica. Section D, Biological crystallography. Biological crystallography., 2004-07, Vol.60 (7), p.1214-1219
Main Authors: Yun, Cai-Hong, Bai, Juan, Sun, Da-Ye, Cui, Da-Fu, Chang, Wen-Rui, Liang, Dong-Cai
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Calmodulin - chemistry
Hydrophobic and Hydrophilic Interactions
Models, Molecular
Molecular Sequence Data
PCM6
plant calmodulin
Protein Structure, Tertiary
Sequence Alignment
Solanum tuberosum - chemistry
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Summary:The crystal structure of a potato calmodulin (PCM6) was solved by molecular replacement and refined to a crystallographic R factor of 22.8% (Rfree = 25.0%) using X‐­ray diffraction data in the resolution range 8.0–2.0 Å. This is the first report of the three‐dimensional structure of a plant Ca2+‐calmodulin. PCM6 crystallizes in a crystal form that belongs to space group P212121, which is different to that of most other calmodulin crystals. The main structural difference between PCM6 and the other calmodulins is in the central helix region and appears to be caused by crystal packing. The surface properties of PCM6 molecules were compared with those of animal calmodulins, which provided an explanation for the unique crystal‐packing state of PCM6.
ISSN:1399-0047
0907-4449
1399-0047
DOI:10.1107/S0907444904009771