Peptides and multiple antigen peptides from Schistosoma mansoni glyceraldehyde 3-phosphate dehydrogenase: preparation, immunogenicity and immunoprotective capacity in C57BL/6 mice

Four monoepitopic MAPs (MAP A, B, C and E) and one bis‐diepitopic MAP B‐E derived from the primary sequence of Schistosoma mansoni glyceraldehyde 3‐phosphate dehydrogenase, previously tested in BALB/c mice, were examined for their immunogenicity and protective capacity in C57BL/6 mice. Despite multi...

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Bibliographic Details
Published in:Journal of peptide science 2004-06, Vol.10 (6), p.350-362
Main Authors: Vepr̆ek, Pavel, Jez̆ek, Jan, Velek, Jir̆í, Tallima, Hatem, Montash, Mona, Ridi, Rashika El
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Antibodies, Helminth - immunology
Antigens, Helminth - chemistry
Antigens, Helminth - immunology
Cytokines - immunology
Epitopes - chemistry
Epitopes - immunology
Female
Glyceraldehyde-3-Phosphate Dehydrogenases - chemistry
Glyceraldehyde-3-Phosphate Dehydrogenases - immunology
Mice
Mice, Inbred C57BL
Molecular Sequence Data
multiple antigen peptides (MAPs)
peptide vaccine
Peptides - chemical synthesis
Peptides - chemistry
Peptides - immunology
RP-HPLC purification
Schistosoma mansoni
Schistosoma mansoni - enzymology
Schistosoma mansoni - immunology
Schistosomiasis mansoni - prevention & control
SG3PDH
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Summary:Four monoepitopic MAPs (MAP A, B, C and E) and one bis‐diepitopic MAP B‐E derived from the primary sequence of Schistosoma mansoni glyceraldehyde 3‐phosphate dehydrogenase, previously tested in BALB/c mice, were examined for their immunogenicity and protective capacity in C57BL/6 mice. Despite multimerization into MAPs, MAP A and MAP C were poorly immunogenic. In contrast to BALB/c mice, MAP E was non‐immunogenic in C57BL/6 mice. Peptide B in the form of MAP B or bis‐diepitopic MAP B‐E elicited immune responses in C57BL/6 mice that were associated with a significant decrease in worm burden. The MAPs were prepared by the stepwise solid‐phase peptide synthesis using Boc/Bzl chemistry, successfully purified on the RP‐HPLC column and characterized by RP‐HPLC, HPCE and MALDI‐TOF MS techniques. A general strategy for MAPs purification is discussed here and the purification of MAP B and MAP E is documented in detail. Copyright © 2004 European Peptide Society and John Wiley & Sons, Ltd.
ISSN:1075-2617
1099-1387
DOI:10.1002/psc.550