Detection of 14 alleles derived from the MHC class I A locus in cynomolgus monkeys

A basic understanding of the major histocompatibility complex (MHC) class I, which, together with T-cell receptors, is a key player in antigen recognition by cytotoxic T lymphocytes, is necessary to study the cellular immune response to intracellular pathogens. The MHC has hardly been reported in cy...

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Bibliographic Details
Published in:Immunogenetics (New York) 2004-06, Vol.56 (3), p.155-163
Main Authors: Uda, Akihiko, Tanabayashi, Kiyoshi, Yamada, Yasuko K, Akari, Hirofumi, Lee, Young-Jung, Mukai, Ryozaburo, Terao, Keiji, Yamada, Akio
Format: Article
Language:English
Subjects:
Alleles
Amino Acid Sequence
Animal models
Animals
Base Sequence
Female
Genes, MHC Class I
Histocompatibility Antigens Class I - chemistry
Histocompatibility Antigens Class I - genetics
Histocompatibility Antigens Class I - metabolism
Immune response
Lymphocytes
Macaca fascicularis - genetics
Male
Metabolism
Molecular Sequence Data
Pedigree
Peptides
Phylogeny
Polymerase Chain Reaction
Sequence Alignment
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Summary:A basic understanding of the major histocompatibility complex (MHC) class I, which, together with T-cell receptors, is a key player in antigen recognition by cytotoxic T lymphocytes, is necessary to study the cellular immune response to intracellular pathogens. The MHC has hardly been reported in cynomolgus monkeys ( Macaca facicularis), although cynomolgus monkeys have been frequently used as the surrogate animal model. We attempted to determine the nucleotide sequences of the MHC class I A locus of cynomolgus monkeys ( Mafa-A) and eventually 34 independent sequences of Mafa-A were obtained from 29 cynomolgus monkeys. These 34 sequences were classified into 14 Mafa-A alleles according to the results of phylogenetic analyses using the neighbor-joining method. One to three Mafa-A alleles were obtained from a single animal. We also tried to establish a multiplex PCR-SSP method for convenient typing of Mafa-A alleles. cDNA from a family of cynomolgus monkeys, which is composed of four sirs and four dams, were examined by multiplex PCR-SSP. The result of multiplex PCR-SSP showed that an individual cynomolgus monkey had two or three Mafa-A alleles, suggesting that the A locus of cynomolgus monkeys might be duplicated.
ISSN:0093-7711
1432-1211
DOI:10.1007/s00251-004-0683-0