Loading…
Bone marrow stroma in childhood myelodysplastic syndrome: composition, ability to sustain hematopoiesis in vitro, and altered gene expression
We studied bone marrow stromal cell cultures from patients with childhood myelodysplastic syndromes (MDS, refractory anemia with excess of blasts, RAEB) and from matched normal donors. Stromal cell monolayers were characterized as myofibroblasts by the expression of smooth muscle α-actin, collagen I...
Saved in:
Published in: | Leukemia research 2004-08, Vol.28 (8), p.831-844 |
---|---|
Main Authors: | , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
cited_by | cdi_FETCH-LOGICAL-c392t-89f20b629042088b200c82c4346cd308fb629445c024b50aaaebe425736f778f3 |
---|---|
cites | cdi_FETCH-LOGICAL-c392t-89f20b629042088b200c82c4346cd308fb629445c024b50aaaebe425736f778f3 |
container_end_page | 844 |
container_issue | 8 |
container_start_page | 831 |
container_title | Leukemia research |
container_volume | 28 |
creator | Borojevic, Radovan Roela, Rosimeire A Rodarte, Renato S Thiago, Leandro S Pasini, Fátima S Conti, Fabiana M Rossi, Maria Isabel D Reis, Luiz F.L Lopes, Luiz F Brentani, M.Mitzi |
description | We studied bone marrow stromal cell cultures from patients with childhood myelodysplastic syndromes (MDS, refractory anemia with excess of blasts, RAEB) and from matched normal donors. Stromal cell monolayers were characterized as myofibroblasts by the expression of smooth muscle α-actin, collagen IV, laminin and fibronectin. When normal cord blood cells were plated onto myelodysplastic stromas, a pathologic cell differentiation was observed, indicating altered myelosupportive properties. cDNA array analysis showed that patient stromas expressed increased levels of thrombospondin-1, collagen-I α2-chain, osteoblast-specific factor-2 and osteonectin, indicating the presence of increased osteoblast content, as confirmed by enhanced alkaline phosphatase synthesis. Alterations in the myelodysplastic stroma environment might contribute to abnormal hematopoiesis in this pathology. |
doi_str_mv | 10.1016/j.leukres.2003.11.019 |
format | article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_66647856</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0145212603004156</els_id><sourcerecordid>66647856</sourcerecordid><originalsourceid>FETCH-LOGICAL-c392t-89f20b629042088b200c82c4346cd308fb629445c024b50aaaebe425736f778f3</originalsourceid><addsrcrecordid>eNqFkU9v1DAQxS0EokvhI4B84tQE24kThwuCin9SpV7K2XLsCesliYPHKc2H4Dvj1a7Esac5zG_e07xHyGvOSs548-5QjrD-ioClYKwqOS8Z756QHVdtVUhVyadkx3gtC8FFc0FeIB4YY7Lj3XNywaVglVB8R_5-CjPQycQY_lBMMUyG-pnavR_dPgRHpw3G4DZcRoPJW4rb7DIF76kN0xLQJx_mK2p6P_q00RQorphM1tjDZFJYggf0eBS991k_o7OjZkwQwdGfkN3hYcl_YNZ5SZ4NZkR4dZ6X5MeXz3fX34qb26_frz_eFLbqRCpUNwjWN6JjtWBK9TkBq4Stq7qxrmJqOO7qWlom6l4yYwz0UAvZVs3QtmqoLsnbk-4Sw-8VMOnJo4VxNDOEFXXTNHWrZPMoyNtOdTnUDMoTaGNAjDDoJfoc66Y508fC9EGfC9PHwjTnOheW796cDdZ-Avf_6txQBj6cAMh53HuIGq2H2YLzEWzSLvhHLP4BjGGssQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17989591</pqid></control><display><type>article</type><title>Bone marrow stroma in childhood myelodysplastic syndrome: composition, ability to sustain hematopoiesis in vitro, and altered gene expression</title><source>ScienceDirect Freedom Collection 2022-2024</source><creator>Borojevic, Radovan ; Roela, Rosimeire A ; Rodarte, Renato S ; Thiago, Leandro S ; Pasini, Fátima S ; Conti, Fabiana M ; Rossi, Maria Isabel D ; Reis, Luiz F.L ; Lopes, Luiz F ; Brentani, M.Mitzi</creator><creatorcontrib>Borojevic, Radovan ; Roela, Rosimeire A ; Rodarte, Renato S ; Thiago, Leandro S ; Pasini, Fátima S ; Conti, Fabiana M ; Rossi, Maria Isabel D ; Reis, Luiz F.L ; Lopes, Luiz F ; Brentani, M.Mitzi</creatorcontrib><description>We studied bone marrow stromal cell cultures from patients with childhood myelodysplastic syndromes (MDS, refractory anemia with excess of blasts, RAEB) and from matched normal donors. Stromal cell monolayers were characterized as myofibroblasts by the expression of smooth muscle α-actin, collagen IV, laminin and fibronectin. When normal cord blood cells were plated onto myelodysplastic stromas, a pathologic cell differentiation was observed, indicating altered myelosupportive properties. cDNA array analysis showed that patient stromas expressed increased levels of thrombospondin-1, collagen-I α2-chain, osteoblast-specific factor-2 and osteonectin, indicating the presence of increased osteoblast content, as confirmed by enhanced alkaline phosphatase synthesis. Alterations in the myelodysplastic stroma environment might contribute to abnormal hematopoiesis in this pathology.</description><identifier>ISSN: 0145-2126</identifier><identifier>EISSN: 1873-5835</identifier><identifier>DOI: 10.1016/j.leukres.2003.11.019</identifier><identifier>PMID: 15203281</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Actins - metabolism ; Alkaline Phosphatase - metabolism ; Anemia, Refractory, with Excess of Blasts ; Bone Marrow - metabolism ; Bone Marrow - pathology ; Bone marrow stromal cells ; Case-Control Studies ; cDNA array ; Cell Differentiation ; Child ; Child, Preschool ; Childhood myelodysplastic syndrome ; Collagen Type IV - metabolism ; Environment ; Female ; Fetal Blood - chemistry ; Fetal Blood - metabolism ; Fibroblasts - pathology ; Fibronectins - metabolism ; Gene expression ; Gene Expression Profiling ; Gene Expression Regulation, Neoplastic ; Hematopoiesis ; Humans ; In Vitro Techniques ; Infant ; Laminin - metabolism ; Male ; Muscle, Smooth - metabolism ; Muscle, Smooth - pathology ; Myelodysplastic Syndromes - genetics ; Myelodysplastic Syndromes - metabolism ; Myelodysplastic Syndromes - pathology ; Oligonucleotide Array Sequence Analysis ; Osteoblasts - metabolism ; Preleukemia ; Stromal Cells - metabolism ; Stromal Cells - pathology</subject><ispartof>Leukemia research, 2004-08, Vol.28 (8), p.831-844</ispartof><rights>2004 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c392t-89f20b629042088b200c82c4346cd308fb629445c024b50aaaebe425736f778f3</citedby><cites>FETCH-LOGICAL-c392t-89f20b629042088b200c82c4346cd308fb629445c024b50aaaebe425736f778f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15203281$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Borojevic, Radovan</creatorcontrib><creatorcontrib>Roela, Rosimeire A</creatorcontrib><creatorcontrib>Rodarte, Renato S</creatorcontrib><creatorcontrib>Thiago, Leandro S</creatorcontrib><creatorcontrib>Pasini, Fátima S</creatorcontrib><creatorcontrib>Conti, Fabiana M</creatorcontrib><creatorcontrib>Rossi, Maria Isabel D</creatorcontrib><creatorcontrib>Reis, Luiz F.L</creatorcontrib><creatorcontrib>Lopes, Luiz F</creatorcontrib><creatorcontrib>Brentani, M.Mitzi</creatorcontrib><title>Bone marrow stroma in childhood myelodysplastic syndrome: composition, ability to sustain hematopoiesis in vitro, and altered gene expression</title><title>Leukemia research</title><addtitle>Leuk Res</addtitle><description>We studied bone marrow stromal cell cultures from patients with childhood myelodysplastic syndromes (MDS, refractory anemia with excess of blasts, RAEB) and from matched normal donors. Stromal cell monolayers were characterized as myofibroblasts by the expression of smooth muscle α-actin, collagen IV, laminin and fibronectin. When normal cord blood cells were plated onto myelodysplastic stromas, a pathologic cell differentiation was observed, indicating altered myelosupportive properties. cDNA array analysis showed that patient stromas expressed increased levels of thrombospondin-1, collagen-I α2-chain, osteoblast-specific factor-2 and osteonectin, indicating the presence of increased osteoblast content, as confirmed by enhanced alkaline phosphatase synthesis. Alterations in the myelodysplastic stroma environment might contribute to abnormal hematopoiesis in this pathology.</description><subject>Actins - metabolism</subject><subject>Alkaline Phosphatase - metabolism</subject><subject>Anemia, Refractory, with Excess of Blasts</subject><subject>Bone Marrow - metabolism</subject><subject>Bone Marrow - pathology</subject><subject>Bone marrow stromal cells</subject><subject>Case-Control Studies</subject><subject>cDNA array</subject><subject>Cell Differentiation</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Childhood myelodysplastic syndrome</subject><subject>Collagen Type IV - metabolism</subject><subject>Environment</subject><subject>Female</subject><subject>Fetal Blood - chemistry</subject><subject>Fetal Blood - metabolism</subject><subject>Fibroblasts - pathology</subject><subject>Fibronectins - metabolism</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Hematopoiesis</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Infant</subject><subject>Laminin - metabolism</subject><subject>Male</subject><subject>Muscle, Smooth - metabolism</subject><subject>Muscle, Smooth - pathology</subject><subject>Myelodysplastic Syndromes - genetics</subject><subject>Myelodysplastic Syndromes - metabolism</subject><subject>Myelodysplastic Syndromes - pathology</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Osteoblasts - metabolism</subject><subject>Preleukemia</subject><subject>Stromal Cells - metabolism</subject><subject>Stromal Cells - pathology</subject><issn>0145-2126</issn><issn>1873-5835</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNqFkU9v1DAQxS0EokvhI4B84tQE24kThwuCin9SpV7K2XLsCesliYPHKc2H4Dvj1a7Esac5zG_e07xHyGvOSs548-5QjrD-ioClYKwqOS8Z756QHVdtVUhVyadkx3gtC8FFc0FeIB4YY7Lj3XNywaVglVB8R_5-CjPQycQY_lBMMUyG-pnavR_dPgRHpw3G4DZcRoPJW4rb7DIF76kN0xLQJx_mK2p6P_q00RQorphM1tjDZFJYggf0eBS991k_o7OjZkwQwdGfkN3hYcl_YNZ5SZ4NZkR4dZ6X5MeXz3fX34qb26_frz_eFLbqRCpUNwjWN6JjtWBK9TkBq4Stq7qxrmJqOO7qWlom6l4yYwz0UAvZVs3QtmqoLsnbk-4Sw-8VMOnJo4VxNDOEFXXTNHWrZPMoyNtOdTnUDMoTaGNAjDDoJfoc66Y508fC9EGfC9PHwjTnOheW796cDdZ-Avf_6txQBj6cAMh53HuIGq2H2YLzEWzSLvhHLP4BjGGssQ</recordid><startdate>20040801</startdate><enddate>20040801</enddate><creator>Borojevic, Radovan</creator><creator>Roela, Rosimeire A</creator><creator>Rodarte, Renato S</creator><creator>Thiago, Leandro S</creator><creator>Pasini, Fátima S</creator><creator>Conti, Fabiana M</creator><creator>Rossi, Maria Isabel D</creator><creator>Reis, Luiz F.L</creator><creator>Lopes, Luiz F</creator><creator>Brentani, M.Mitzi</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20040801</creationdate><title>Bone marrow stroma in childhood myelodysplastic syndrome: composition, ability to sustain hematopoiesis in vitro, and altered gene expression</title><author>Borojevic, Radovan ; Roela, Rosimeire A ; Rodarte, Renato S ; Thiago, Leandro S ; Pasini, Fátima S ; Conti, Fabiana M ; Rossi, Maria Isabel D ; Reis, Luiz F.L ; Lopes, Luiz F ; Brentani, M.Mitzi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c392t-89f20b629042088b200c82c4346cd308fb629445c024b50aaaebe425736f778f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Actins - metabolism</topic><topic>Alkaline Phosphatase - metabolism</topic><topic>Anemia, Refractory, with Excess of Blasts</topic><topic>Bone Marrow - metabolism</topic><topic>Bone Marrow - pathology</topic><topic>Bone marrow stromal cells</topic><topic>Case-Control Studies</topic><topic>cDNA array</topic><topic>Cell Differentiation</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Childhood myelodysplastic syndrome</topic><topic>Collagen Type IV - metabolism</topic><topic>Environment</topic><topic>Female</topic><topic>Fetal Blood - chemistry</topic><topic>Fetal Blood - metabolism</topic><topic>Fibroblasts - pathology</topic><topic>Fibronectins - metabolism</topic><topic>Gene expression</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Hematopoiesis</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Infant</topic><topic>Laminin - metabolism</topic><topic>Male</topic><topic>Muscle, Smooth - metabolism</topic><topic>Muscle, Smooth - pathology</topic><topic>Myelodysplastic Syndromes - genetics</topic><topic>Myelodysplastic Syndromes - metabolism</topic><topic>Myelodysplastic Syndromes - pathology</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Osteoblasts - metabolism</topic><topic>Preleukemia</topic><topic>Stromal Cells - metabolism</topic><topic>Stromal Cells - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Borojevic, Radovan</creatorcontrib><creatorcontrib>Roela, Rosimeire A</creatorcontrib><creatorcontrib>Rodarte, Renato S</creatorcontrib><creatorcontrib>Thiago, Leandro S</creatorcontrib><creatorcontrib>Pasini, Fátima S</creatorcontrib><creatorcontrib>Conti, Fabiana M</creatorcontrib><creatorcontrib>Rossi, Maria Isabel D</creatorcontrib><creatorcontrib>Reis, Luiz F.L</creatorcontrib><creatorcontrib>Lopes, Luiz F</creatorcontrib><creatorcontrib>Brentani, M.Mitzi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Leukemia research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Borojevic, Radovan</au><au>Roela, Rosimeire A</au><au>Rodarte, Renato S</au><au>Thiago, Leandro S</au><au>Pasini, Fátima S</au><au>Conti, Fabiana M</au><au>Rossi, Maria Isabel D</au><au>Reis, Luiz F.L</au><au>Lopes, Luiz F</au><au>Brentani, M.Mitzi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bone marrow stroma in childhood myelodysplastic syndrome: composition, ability to sustain hematopoiesis in vitro, and altered gene expression</atitle><jtitle>Leukemia research</jtitle><addtitle>Leuk Res</addtitle><date>2004-08-01</date><risdate>2004</risdate><volume>28</volume><issue>8</issue><spage>831</spage><epage>844</epage><pages>831-844</pages><issn>0145-2126</issn><eissn>1873-5835</eissn><abstract>We studied bone marrow stromal cell cultures from patients with childhood myelodysplastic syndromes (MDS, refractory anemia with excess of blasts, RAEB) and from matched normal donors. Stromal cell monolayers were characterized as myofibroblasts by the expression of smooth muscle α-actin, collagen IV, laminin and fibronectin. When normal cord blood cells were plated onto myelodysplastic stromas, a pathologic cell differentiation was observed, indicating altered myelosupportive properties. cDNA array analysis showed that patient stromas expressed increased levels of thrombospondin-1, collagen-I α2-chain, osteoblast-specific factor-2 and osteonectin, indicating the presence of increased osteoblast content, as confirmed by enhanced alkaline phosphatase synthesis. Alterations in the myelodysplastic stroma environment might contribute to abnormal hematopoiesis in this pathology.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>15203281</pmid><doi>10.1016/j.leukres.2003.11.019</doi><tpages>14</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0145-2126 |
ispartof | Leukemia research, 2004-08, Vol.28 (8), p.831-844 |
issn | 0145-2126 1873-5835 |
language | eng |
recordid | cdi_proquest_miscellaneous_66647856 |
source | ScienceDirect Freedom Collection 2022-2024 |
subjects | Actins - metabolism Alkaline Phosphatase - metabolism Anemia, Refractory, with Excess of Blasts Bone Marrow - metabolism Bone Marrow - pathology Bone marrow stromal cells Case-Control Studies cDNA array Cell Differentiation Child Child, Preschool Childhood myelodysplastic syndrome Collagen Type IV - metabolism Environment Female Fetal Blood - chemistry Fetal Blood - metabolism Fibroblasts - pathology Fibronectins - metabolism Gene expression Gene Expression Profiling Gene Expression Regulation, Neoplastic Hematopoiesis Humans In Vitro Techniques Infant Laminin - metabolism Male Muscle, Smooth - metabolism Muscle, Smooth - pathology Myelodysplastic Syndromes - genetics Myelodysplastic Syndromes - metabolism Myelodysplastic Syndromes - pathology Oligonucleotide Array Sequence Analysis Osteoblasts - metabolism Preleukemia Stromal Cells - metabolism Stromal Cells - pathology |
title | Bone marrow stroma in childhood myelodysplastic syndrome: composition, ability to sustain hematopoiesis in vitro, and altered gene expression |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T09%3A53%3A15IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Bone%20marrow%20stroma%20in%20childhood%20myelodysplastic%20syndrome:%20composition,%20ability%20to%20sustain%20hematopoiesis%20in%20vitro,%20and%20altered%20gene%20expression&rft.jtitle=Leukemia%20research&rft.au=Borojevic,%20Radovan&rft.date=2004-08-01&rft.volume=28&rft.issue=8&rft.spage=831&rft.epage=844&rft.pages=831-844&rft.issn=0145-2126&rft.eissn=1873-5835&rft_id=info:doi/10.1016/j.leukres.2003.11.019&rft_dat=%3Cproquest_cross%3E66647856%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c392t-89f20b629042088b200c82c4346cd308fb629445c024b50aaaebe425736f778f3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=17989591&rft_id=info:pmid/15203281&rfr_iscdi=true |