Effect of flax supplementation and growth promotants on lipoprotein lipase and glycogenin messenger RNA concentrations in finishing cattle

• Published in: Journal of animal science 2004-06, Vol.82 (6), p.1868-1875
• Main Authors: Waylan, A.T, Dunn, J.D, Johnson, B.J, Kayser, J.P, Sissom, E.K
• Format: Article
• Language: English
• Subjects: alpha-Linolenic Acid - metabolism; Animal productions; Animals; Biological and medical sciences; Cattle - growth & development; Cattle - metabolism; Dietary Supplements; Dose-Response Relationship, Drug; Drug Combinations; Drug Implants; Estradiol - administration & dosage; Estradiol - pharmacology; Flax; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation; Glucosyltransferases; Glycoproteins - genetics; Glycoproteins - metabolism; Lipoprotein Lipase - genetics; Lipoprotein Lipase - metabolism; Male; Muscle, Skeletal - metabolism; Polymerase Chain Reaction - veterinary; Random Allocation; RNA, Messenger - analysis; RNA, Messenger - metabolism; Terrestrial animal productions; Trenbolone Acetate - administration & dosage; Trenbolone Acetate - analogs & derivatives; Trenbolone Acetate - pharmacology; Vertebrates
• ISSN: 0021-8812; 1525-3163
• DOI: 10.2527/2004.8261868x

Lipoprotein lipase (LPL) hydrolyzes triacylglycerols into monoacylglycerol and fatty acids, which are taken up by tissues and used for energy. Glycogenin is the core protein on which glycogen molecules are synthesized. There is one molecule of glycogenin per molecule of glycogen in skeletal muscle; therefore, glycogen storage is limited by the amount of glycogenin present in muscle. The objective of this study was to investigate the effect of feeding flaxseed, a source of PUFA, and administering a growth promoter on steady-state LPL and glycogenin mRNA content of muscle in finishing cattle. Sixteen crossbred steers (initial BW = 397 kg), given ad libitum access to a 92% concentrate diet for 28 d, were used in a four-treatment, 2 x 2 factorial experiment, with flaxseed supplementation (0 or 5% of dietary DM) and implanting (not implanted or implanted with Revalor-S) as the main effects. Muscle biopsies were obtained from the LM at 0, 14, and 28 d, and used to quantify LPL and glycogenin mRNA concentrations using real-time quantitative PCR. Implanting with Revalor-S did not affect LPL (P = 0.13) or glycogenin (P = 0.98) mRNA concentrations. A day x flaxseed interaction (P < 0.001) was observed for both LPL and glycogenin mRNA concentrations. No differences (P > 0.10) were observed between 0 and 5% flaxseed supplemented steers; however, at 28 d, nonflaxseed-fed steers had 4.1- and 5.7-fold increases (P < 0.001) over flaxseed steers for LPL and glycogenin mRNA concentrations, respectively. To further evaluate the effects of alpha-linolenic acid (alpha-LA) on LPL and glycogenin mRNA concentrations, muscle satellite cells were isolated from five finishing steers, and different alpha-LA concentrations were applied in culture. The RNA was isolated from the bovine satellite cells. Addition of alpha-LA numerically increased (P = 0.16) the LPL mRNA concentration 48% at 1 micromolar alpha-LA compared with the control. The expression of glycogenin was increased (P < 0.05) 50% at 1 micromolar alpha-LA compared with the control. These results suggest that flaxseed supplementation to finishing steers for 28 d decreased gene expression of both LPL and glycogenin compared with not feeding flaxseed. Alterations in local concentrations of these two proteins could affect the ability of muscle to use fatty acids and glucose for energy, and, ultimately, affect carcass quality.