Human Histone Deacetylase SIRT2 Interacts with the Homeobox Transcription Factor HOXA10

Histone deacetylases are required for transcriptional repression in eukaryotes. Saccharomyces cerevisiae has several histone deacetylases, of which ySir2p is the most conserved throughout evolution. Currently, there is no report on the interacting protein partner of a human Sir2 homolog, SIRT2. Here...

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Bibliographic Details
Published in:Journal of biochemistry (Tokyo) 2004-06, Vol.135 (6), p.695-700
Main Authors: Bae, Nancy S., Swanson, Mark J., Vassilev, Alex, Howard, Bruce H.
Format: Article
Language:English
Subjects:
Animals
Cell Line
Cell-Free System
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
Homeodomain Proteins
HOXA10
Humans
Mice
Protein Binding
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - metabolism
SIRT2
Sirtuin 2
Sirtuins - genetics
Sirtuins - metabolism
Two-Hybrid System Techniques
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Summary:Histone deacetylases are required for transcriptional repression in eukaryotes. Saccharomyces cerevisiae has several histone deacetylases, of which ySir2p is the most conserved throughout evolution. Currently, there is no report on the interacting protein partner of a human Sir2 homolog, SIRT2. Here we show for the first time that SIRT2 interacts with the homeobox transcription factor, HOXA10, which was identified in a two-hybrid screen. Interactions were confirmed by co-immunoprecipitation from in vitro translations as well as in human cell-free extracts. Taken together with mouse knockout studies, our results raise the intriguing possibility that SIRT2 plays a role in mammalian development.
ISSN:0021-924X
DOI:10.1093/jb/mvh084