Intracellular expression of Vitreoscilla hemoglobin in Aspergillus terreus to alleviate the effect of a short break in aeration during culture

A continuous supply of O2 is important for itaconic acid production in Aspergillus terreus. Any interruption of aeration significantly reduces itaconic acid production. To overcome this effect, A. terreus M8 was transformed with the Vitreoscilla hemoglobin gene (vgb) which, as shown by Southern hybr...

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Bibliographic Details
Published in:Biotechnology letters 2004-07, Vol.26 (13), p.1067-1072
Main Authors: Lin, Y.H, Li, Y.F, Huang, M.C, Tsai, Y.C
Format: Article
Language:English
Subjects:
Acid production
Aeration
Aspergillus - metabolism
Aspergillus terreus
Bacteria
Bacterial Proteins - metabolism
Base Sequence
Biological and medical sciences
biological production
Biotechnology
Blotting, Southern
cell culture
Chromosomes, Fungal
dicarboxylic acids
DNA Primers
Fundamental and applied biological sciences. Psychology
genetic transformation
Hemoglobins - metabolism
itaconic acid
Oxygen - metabolism
Polymerase Chain Reaction
Succinates - metabolism
Truncated Hemoglobins
Vitreoscilla
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Summary:A continuous supply of O2 is important for itaconic acid production in Aspergillus terreus. Any interruption of aeration significantly reduces itaconic acid production. To overcome this effect, A. terreus M8 was transformed with the Vitreoscilla hemoglobin gene (vgb) which, as shown by Southern hybridization, was integrated into the recipient chromosome. The activity of the expressed hemoglobin was confirmed by a CO-difference spectrum. During itaconic acid production, the effect of a break in aeration during cultivation in the transformant with the vgb gene is alleviated. Additionally, the transformant shows improved itaconic acid production.
ISSN:0141-5492
1573-6776
DOI:10.1023/b:bile.0000032964.15178.7c