Genetic line effect on peripheral blood leukocyte cell surface marker expression in chickens

To determine the role of genetics in baseline lymphocyte parameters, several distinct lines of chickens were examined for differences in peripheral blood leukocyte (PBL) populations. Four highly inbred chicken lines (MHC congenic Fayoumi lines M15.2 and M5.1, and MHC congenic Leghorn lines G-B1 and...

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Bibliographic Details
Published in:Poultry science 2004-06, Vol.83 (6), p.911-916
Main Authors: Cheeseman, J.H, Kaiser, M.G, Lamont, S.J
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal
Antigens, Surface - analysis
B-lymphocytes
breed differences
broiler chickens
CD3 Complex - analysis
CD4 Antigens - analysis
CD4-positive T-lymphocytes
CD8 Antigens - analysis
CD8-positive T-lymphocytes
chickens
Chickens - blood
Chickens - genetics
Crosses, Genetic
Flow Cytometry
gene expression
genetic variation
inbred lines
Inbreeding
Leukocyte Count
Leukocytes, Mononuclear - immunology
line differences
Lipopolysaccharide Receptors - analysis
major histocompatibility complex
receptors
surface antigens
T-lymphocytes
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Summary:To determine the role of genetics in baseline lymphocyte parameters, several distinct lines of chickens were examined for differences in peripheral blood leukocyte (PBL) populations. Four highly inbred chicken lines (MHC congenic Fayoumi lines M15.2 and M5.1, and MHC congenic Leghorn lines G-B1 and G-B2), two advanced intercrosses [F5 (Broiler x G-B2) and F5 (Broiler x M15.2)], and an outbred population of broilers were used. Leukocytes isolated from healthy adult birds were labeled with monoclonal antibodies: chCD3, chCD4, chCD8, chBu-1, and hCD14. Flow cytometry was used to determine the total percentage of positively labeled cells for each surface marker in a sample, as well as the mean fluorescent intensity, or surface marker density, of a labeled subset. Significant line differences for percentage positive CD3 T cells and the ratio of B cells:T cells (represented by the Bu-1:CD3 ratio) were found. The effect of line was also significant for CD3 and CD8 T cell receptor density. Effects of sex and MHC on PBL cell surface marker expression were not significant in the lines examined. This study demonstrates the effect of genetic line on resting leukocyte composition of peripheral blood in the chicken lines examined. Observed PBL differences add to our growing knowledge of the varied roles that immune system status (defined by specific cell populations) and genetic background have in determining susceptibility and disease progression in chickens.
ISSN:0032-5791
1525-3171
DOI:10.1093/ps/83.6.911