Intracellular expression of antisense RNA transcripts complementary to the human immunodeficiency virus type-1 vif gene inhibits viral replication in infected T-lymphoblastoid cells

The human immunodeficiency virus type-1 (HIV-1)-encoded vif protein is essential for viral replication, virion production, and pathogenicity. HIV-1 vif interacts with the endogenous human APOBEC3G protein (an mRNA editor) in target cells to prevent its virions from encapsidation. Although some studi...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2004-07, Vol.320 (2), p.544-550
Main Authors: Barnor, Jacob Samson, Miyano-Kurosaki, Naoko, Yamaguchi, Kazuya, Sakamoto, Atsushi, Ishikawa, Koichi, Inagaki, Yoshio, Yamamoto, Naoki, Osei-Kwasi, Mubarak, Ofori-Adjei, David, Takaku, Hiroshi
Format: Article
Language:English
Subjects:
Antisense RNA
Base Sequence
Cell Line
DNA Primers
Down-Regulation
Gene therapy
Genes, vif
HIV-1 - genetics
HIV-1 - physiology
HIV-1 vif
Human immunodeficiency virus 1
Humans
Inhibition of HIV-1 replication
RNA, Antisense - genetics
RNA, Messenger - genetics
T-Lymphocytes - virology
Virus Replication - genetics
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Summary:The human immunodeficiency virus type-1 (HIV-1)-encoded vif protein is essential for viral replication, virion production, and pathogenicity. HIV-1 vif interacts with the endogenous human APOBEC3G protein (an mRNA editor) in target cells to prevent its virions from encapsidation. Although some studies have established targets within the HIV-1 vif gene that are important for its biologic function, it is however important to further screen for effective therapeutic targets in the vif gene that could interfere with the HIV-1 vif-dependent infectivity and pathogenicity. This report demonstrates that HIV-1 vif antisense RNA fragments constructed within mid-3 ′ region, notably the region spanning nucleic acid positions 5561–5705 (M-3 ′-AS), significantly inhibited HIV-1 replication in MT-4 and H9-infected cells and reduced the HIV-1 vif mRNA transcripts. These data clearly suggest that the above vif fragment, which corresponds to amino acid residues 96–144, could be an effective novel therapeutic target site for gene therapy applications, for the control and management of HIV-1 infection, due to its strong inhibition of HIV-1 replication in cells.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2004.05.201