Photoreactivation of Legionella pneumophila after inactivation by low- or medium-pressure ultraviolet lamp

Photoreactivation of Legionella pneumophila after the inactivation by low-pressure (LP) or medium-pressure (MP) UV lamp was investigated in comparison with that of Escherichia coli. An endonuclease sensitive site (ESS) assay was used to determine the number of UV-induced pyrimidine dimers in the gen...

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Bibliographic Details
Published in:Water research (Oxford) 2004-06, Vol.38 (11), p.2757-2763
Main Authors: Oguma, Kumiko, Katayama, Hiroyuki, Ohgaki, Shinichiro
Format: Article
Language:English
Subjects:
Applied sciences
DNA, Bacterial - analysis
Drinking water and swimming-pool water. Desalination
Escherichia coli
Escherichia coli - pathogenicity
Exact sciences and technology
Legionella pneumophila
Legionella pneumophila - pathogenicity
Low-pressure UV lamp
Medium-pressure UV lamp
Photochemistry
Photoreactivation
Pollution
Pressure
Survival Analysis
Ultraviolet disinfection
Ultraviolet Rays
Water Purification - methods
Water treatment and pollution
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Summary:Photoreactivation of Legionella pneumophila after the inactivation by low-pressure (LP) or medium-pressure (MP) UV lamp was investigated in comparison with that of Escherichia coli. An endonuclease sensitive site (ESS) assay was used to determine the number of UV-induced pyrimidine dimers in the genome DNA of L. pneumophila or E. coli, while the survival ratio of each bacterium was also investigated by cultivation methods. L. pneumophila performed photoreactivation with almost complete repair of pyrimidine dimers associated with the quick recovery of survival ratio. A 3 log inactivation of L. pneumophila by LP or MP UV lamp was, respectively, resulted in 0.5 log or 0.4 log inactivation when photoreactivation was completed. Interestingly, L. pneumophila performed equivalent photoreactivation after LP and MP UV lamp exposures while photoreactivation of E. coli was significantly repressed after the inactivation by MP UV lamp. This study indicated that an attention would be required to design and operate a UV disinfection system targeting L. pneumophila. It was further implied that E. coli would not correctly indicate the fate of L. pneumophila in UV disinfection systems when photoreactivation takes place.
ISSN:0043-1354
1879-2448
DOI:10.1016/j.watres.2004.03.024