Determination of bilayer thickness and lipid surface area in unilamellar dimyristoylphosphatidylcholine vesicles from small-angle neutron scattering curves: a comparison of evaluation methods

Small-angle neutron scattering (SANS) experiments were performed on unilamellar 1,2-dimyristoylphosphatidylcholine (DMPC) vesicles prepared in heavy water by extrusion through polycarbonate filters with 500 A pores. The data obtained at 30+/-0.1 degrees C were evaluated using a five-strip function m...

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Bibliographic Details
Published in:European biophysics journal 2004-07, Vol.33 (4), p.328-334
Main Authors: Kucerka, Norbert, Kiselev, Mikhail A, Balgavý, Pavol
Format: Article
Language:English
Subjects:
Algorithms
Computer Simulation
Dimyristoylphosphatidylcholine - chemistry
Experimental data
Heavy water
Lipid Bilayers - chemistry
Liposomes - chemistry
Membrane Fluidity
Models, Chemical
Models, Molecular
Molecular Conformation
Neutron Diffraction - methods
Polar environments
Scattering, Radiation
Studies
Surface area
Surface Properties
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Summary:Small-angle neutron scattering (SANS) experiments were performed on unilamellar 1,2-dimyristoylphosphatidylcholine (DMPC) vesicles prepared in heavy water by extrusion through polycarbonate filters with 500 A pores. The data obtained at 30+/-0.1 degrees C were evaluated using a five-strip function model of the bilayer coherent neutron scattering length density, three different approximate form factors describing scattering from vesicles, and different methods of evaluation of the experimental data. It is shown that the results obtained from the SANS data in the range of scattering vector values 0.0316 A(-1)< q
ISSN:0175-7571
1432-1017
DOI:10.1007/s00249-003-0349-0