Molecular Characterization of Iranian Infectious Bursal Disease Viruses

• Published in: Avian diseases 2008-12, Vol.52 (4), p.665-669
• Main Authors: Razmyar, J, Peighambari, S. M
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Amino acid substitution; Amino acids; Animals; Base Sequence; Birnaviridae Infections - epidemiology; Birnaviridae Infections - veterinary; Birnaviridae Infections - virology; bursa of Fabricius; Chickens; genes; immunosuppression (physiological); infectious bursal disease; Infectious bursal disease virus; Infectious bursal disease virus - classification; Infectious bursal disease virus - genetics; Infectious diseases; Iran; Iran - epidemiology; microbial genetics; molecular conformation; Molecular Sequence Data; Mortality; Nucleotides; Phylogeny; poultry; Poultry Diseases - epidemiology; Poultry Diseases - virology; Research Notes; Reverse Transcriptase Polymerase Chain Reaction; Sequence Alignment - veterinary; Sus; Transcriptional regulatory elements; Vaccination; Viral diseases; Viruses; VP2; VP2 gene; vvIBDV
• ISSN: 0005-2086; 1938-4351
• DOI: 10.1637/8280-031008-Reg.1

This study was conducted to characterize nine infectious bursal disease virus (IBDV) isolates from Iran. A reverse transcriptase–polymerase chain reaction (RT-PCR) procedure was used to amplify a 743-bp fragment of the VP2 gene hypervariable region from IBDV field isolates. Amplified VP2 fragments of the nine IBDV isolates were sequenced and compared with published sequences of IBDV strains from Iran and around the world, and their phylogenetic relationships were analyzed. Three isolates demonstrated close relation to classical attenuated strains of IBDVs, and six isolates showed sequences common in European and Asian strains of very virulent IBDVs (vvIBDVs). Four nucleotide changes—802A, 934A, 940A, and 1366A—were common in all Iranian vvIBDVs except in one isolate. Amino acid sequences of three Iranian vvIBDVs were 100% identical and resembled vvIBDV strains from European (UK661), Asian (HK46, GZ96), and Iranian origins (IR01, SDH1). Some unique amino acid substitutions after major hydrophilic peak A in Iranian vvIBDV field isolates were observed: 231S-L, 231S-P, and 233N-K. Phylogenetic analysis showed that the Iranian vvIBDVs were closely related to European and Asian vvIBDVs. Further comprehensive investigations will provide more information on the distribution, variability, and phylogenetic relationships of different IBDVs isolated in Iran and other parts of the world. Abbreviations: DEPC = diethylpyrocarbonate; IBD = infectious bursal disease; IBDV = infectious bursal disease virus; PCR = polymerase chain reaction; RT = reverse transcriptase; TNE buffer = 10 mM Tris/HCl, 100 mM NaCl, 1 mM ethylenediaminetetraacetic acid, pH 8.0; vv = very virulent Nota de Investigación—Caracterización molecular de virus Iraníes de la enfermedad infecciosa de la bolsa. Este estudio se realizó para caracterizar nueve aislamientos del virus de la enfermedad infecciosa de la bolsa provenientes de Irán. Para amplificar un segmento de 743 pares de bases de la región hipervariable de la proteína viral 2 (por sus siglas en Inglés VP2) perteneciente a aislamientos de campo del virus de la enfermedad infecciosa de la bolsa, se utilizó una prueba de reacción en cadena por la polimerasa transcriptasa reversa. Los fragmentos amplificados de la proteína VP2 de los nueve virus de la enfermedad infecciosa de la bolsa se secuenciaron y compararon con secuencias publicadas de cepas de Irán y de alrededor del mundo analizando sus relaciones filogenéticas. Tres de los aislamientos demo