High resolution DNA flow cytometry of boar sperm cells in identification of boars carrying cytogenetic aberrations

The cytogenetic quality of boars used for breeding determines the litter outcome and thus has large economical consequences. Traditionally, quality controls based on the examination of simple karyograms are time consuming and sometimes give uncertain results. As an alternative, the use of high-resol...

Full description

Saved in:
Bibliographic Details
Published in:Theriogenology 2004-08, Vol.62 (3-4), p.501-511
Main Authors: Larsen, Jacob, Christensen, Knud, Larsen, Jørgen K, Jensen, Peter Østrup, Gustavsson, Ingemar, Christensen, Ib Jarle
Format: Article
Language:English
Subjects:
Animals
boars
Chromosome Aberrations
chromosome translocation
Cytogenetics
DNA
DNA - analysis
DNA content
Flow cytometry
Flow Cytometry - methods
Flow Cytometry - veterinary
Fluorescent Dyes
Indoles
karyotyping
Karyotyping - veterinary
Male
male fertility
screening
sire evaluation
Sperm cells
spermatozoa
Spermatozoa - chemistry
Swine
Translocations
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The cytogenetic quality of boars used for breeding determines the litter outcome and thus has large economical consequences. Traditionally, quality controls based on the examination of simple karyograms are time consuming and sometimes give uncertain results. As an alternative, the use of high-resolution DNA flow cytometry on DAPI-stained sperm cell nuclei (CV≤1.3%) was investigated as a screening method for the cytogenetic quality of boars. By analyzing a series of 25 animals judged normal by their fertility statistics and a series of seven animals with known reciprocal translocations, a model for identifying sperm cells from cytogenetically aberrant animals was proposed. This model was applied to a series of 50 uncharacterized animals. The model successfully identified a mosaic or chimaeric carrier of an aberrant X chromosome. However, implementation of this technique for screening purposes would necessitate essential improvements in standardization and measurement precision.
ISSN:0093-691X
1879-3231
DOI:10.1016/j.theriogenology.2003.11.008