Identification of an evolutionarily divergent U11 small nuclear ribonucleoprotein particle in Drosophila

Previous reports suggested that U11, in contrast to U12 or other small nuclear (sn)RNAs of the U12-type spliceosome, might be either highly divergent or absent in Drosophila melanogaster. Affinity purification of Drosophila U12-containing complexes has led to the identification of the fly U11 snRNA,...

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Published in:Proceedings of the National Academy of Sciences - PNAS 2004-06, Vol.101 (26), p.9584-9589
Main Authors: Schneider, C, Will, C.L, Brosius, J, Frilander, M.J, Luhrmann, R
Format: Article
Language:English
Subjects:
Animals
Arabidopsis - chemistry
Arabidopsis - genetics
Base Sequence
Biochemistry
Biological Sciences
Drosophila
Drosophila melanogaster - chemistry
Drosophila melanogaster - genetics
Drosophila Proteins - chemistry
Drosophila Proteins - genetics
Evolution
Evolution, Molecular
Genes
Humans
Insects
Introns
Molecular Sequence Data
Nucleic Acid Conformation
Nucleotides
Ribonucleoproteins, Small Nuclear - chemistry
Ribonucleoproteins, Small Nuclear - genetics
RNA
RNA Splice Sites - genetics
RNA, Plant - chemistry
RNA, Plant - genetics
RNA, Small Nuclear - chemistry
RNA, Small Nuclear - genetics
Small nuclear ribonucleoproteins
Small nuclear RNA
Spliceosomes
Vertebrates
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Summary:Previous reports suggested that U11, in contrast to U12 or other small nuclear (sn)RNAs of the U12-type spliceosome, might be either highly divergent or absent in Drosophila melanogaster. Affinity purification of Drosophila U12-containing complexes has led to the identification of the fly U11 snRNA, which contains a potential U12-type 5′ splice-site-interacting sequence, but whose sequence and length differs significantly from vertebrate and plant U11. Analysis of U12-type introns revealed an A-rich region directly downstream of Drosophila, but not human, U12-type 5′ splice sites. This finding, coupled with the presence of a highly divergent U11 snRNA, and the apparent absence of Drosophila homologs of human U11 proteins, suggest that U12-type 5′ splice site recognition might be different in flies. A comparison of U11 snRNAs that we have identified from vertebrates, plants, and insects, suggests that an evolutionarily divergent U11 snRNA may be unique to Drosophila and not characteristic of insects in general.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.0403400101