Effects of 17beta-estradiol on in vitro maturation of pig oocytes in protein-free medium

The present study examined the effects of 17beta-estradiol (E2) on in vitro maturation and subsequent in vitro fertilization of pig oocytes matured with or without cumulus cells. When E2 (10 ng/ml) was added to the protein-free maturation medium, the proportions of cumulus-enclosed oocytes that unde...

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Bibliographic Details
Published in:The Journal of reproduction and development 2004-06, Vol.50 (3), p.305-313
Main Authors: Li, Q. (Okayama Univ. (Japan)), Niwa, K, Hunter, M.G
Format: Article
Language:English
Subjects:
Animals
Cell Culture Techniques - methods
Cell Nucleus - metabolism
CULTURE MEDIA
Culture Media - chemistry
Estradiol - analogs & derivatives
Estradiol - pharmacology
Estrogen Antagonists - pharmacology
Estrogens - metabolism
Female
Fulvestrant
IN VITRO EXPERIMENTATION
IN VITRO FERTILIZATION
Male
MATURATION
OESTROGENS
Oocytes - drug effects
Oocytes - metabolism
OVA
Progesterone - metabolism
Spermatozoa - metabolism
SWINE
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Summary:The present study examined the effects of 17beta-estradiol (E2) on in vitro maturation and subsequent in vitro fertilization of pig oocytes matured with or without cumulus cells. When E2 (10 ng/ml) was added to the protein-free maturation medium, the proportions of cumulus-enclosed oocytes that underwent germinal vesicle breakdown and reached metaphase II were significantly reduced (P
ISSN:0916-8818
1348-4400
DOI:10.1262/jrd.50.305