Effects of 17beta-estradiol on in vitro maturation of pig oocytes in protein-free medium

The present study examined the effects of 17beta-estradiol (E2) on in vitro maturation and subsequent in vitro fertilization of pig oocytes matured with or without cumulus cells. When E2 (10 ng/ml) was added to the protein-free maturation medium, the proportions of cumulus-enclosed oocytes that unde...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of reproduction and development 2004-06, Vol.50 (3), p.305-313
Main Authors: Li, Q. (Okayama Univ. (Japan)), Niwa, K, Hunter, M.G
Format: Article
Language:English
Subjects:
Animals
Cell Culture Techniques - methods
Cell Nucleus - metabolism
CULTURE MEDIA
Culture Media - chemistry
Estradiol - analogs & derivatives
Estradiol - pharmacology
Estrogen Antagonists - pharmacology
Estrogens - metabolism
Female
Fulvestrant
IN VITRO EXPERIMENTATION
IN VITRO FERTILIZATION
Male
MATURATION
OESTROGENS
Oocytes - drug effects
Oocytes - metabolism
OVA
Progesterone - metabolism
Spermatozoa - metabolism
SWINE
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c2455-4ad24ab8ba56874386de0e11b08a593dca059e17b32c964aa0e9ec49f518310e3
cites cdi_FETCH-LOGICAL-c2455-4ad24ab8ba56874386de0e11b08a593dca059e17b32c964aa0e9ec49f518310e3
container_end_page 313
container_issue 3
container_start_page 305
container_title The Journal of reproduction and development
container_volume 50
creator Li, Q. (Okayama Univ. (Japan))
Niwa, K
Hunter, M.G
description The present study examined the effects of 17beta-estradiol (E2) on in vitro maturation and subsequent in vitro fertilization of pig oocytes matured with or without cumulus cells. When E2 (10 ng/ml) was added to the protein-free maturation medium, the proportions of cumulus-enclosed oocytes that underwent germinal vesicle breakdown and reached metaphase II were significantly reduced (P
doi_str_mv 10.1262/jrd.50.305
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_66667007</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>66667007</sourcerecordid><originalsourceid>FETCH-LOGICAL-c2455-4ad24ab8ba56874386de0e11b08a593dca059e17b32c964aa0e9ec49f518310e3</originalsourceid><addsrcrecordid>eNpF0M9LwzAUB_AgipvTi3elJw9C58uvNj3KmL8Y6EHBW0jb15HRNjNphf33ZmxgLg_Ch8f3fQm5pjCnLGMPG1_PJcw5yBMypVyoVAiAUzKFgmapUlRNyEUIGwDOZCbOyYRKxjJZyCn5XjYNVkNIXJPQvMTBpBgGb2rr2sT1ie2TXzt4l3RmGL0ZbPyLdGvXiXPVbsCwJ1vvBrR92njEpMPajt0lOWtMG_DqOGfk62n5uXhJV-_Pr4vHVVoxIWUqTM2EKVVpZKZywVVWIyClJSgjC15XBmSBMRlnVZEJYwALrETRSKo4BeQzcnfYGzP8jDG77myosG1Nj24MOosvB8gjvD_AyrsQPDZ6621n_E5T0PsedexRS9Cxx4hvj1vHMt7zT4_FRXBzAI1x2qy9DfrtgwFIABoB_wOaMnZs</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>66667007</pqid></control><display><type>article</type><title>Effects of 17beta-estradiol on in vitro maturation of pig oocytes in protein-free medium</title><source>Directory of Open Access Journals</source><creator>Li, Q. (Okayama Univ. (Japan)) ; Niwa, K ; Hunter, M.G</creator><creatorcontrib>Li, Q. (Okayama Univ. (Japan)) ; Niwa, K ; Hunter, M.G</creatorcontrib><description>The present study examined the effects of 17beta-estradiol (E2) on in vitro maturation and subsequent in vitro fertilization of pig oocytes matured with or without cumulus cells. When E2 (10 ng/ml) was added to the protein-free maturation medium, the proportions of cumulus-enclosed oocytes that underwent germinal vesicle breakdown and reached metaphase II were significantly reduced (P&lt;0.05), and cumulus expansion was also significantly inhibited (P&lt;0.05) compared with the control (no E2 added). Although oocytes matured in the presence of E2 were penetrated by sperm in vitro at the same level as the control, the incidences of male pronuclear (MPN) formation and activated oocytes were significantly lower (P&lt;0.05) than the control. These inhibitory effects of E2 were prevented when the medium was supplemented with E2 together with its antagonist, ICI 182,780 (1 micro g/ml), although the presence of the antagonist alone in the medium had no effect on the maturation and fertilization in vitro of oocytes. In cumulus-free oocytes, E2 had no effect on nuclear maturation and penetration in vitro, but low MPN formation was observed in oocytes matured in the presence and absence of E2. When cumulus-enclosed oocytes were cultured in the presence of progesterone (P4; 600 ng/ml) alone or together with E2, no significant differences in nuclear maturation, cumulus expansion or penetration in vitro were observed compared with control oocytes. The concentration of P4 in maturation medium was significantly (P&lt;0.01) lower when cumulus-enclosed oocytes were cultured for 44 h in the medium with E2 than in medium without E2. These results indicate that E2 inhibits both nuclear and cytoplasmic maturation of cumulus-enclosed pig oocytes, and that this inhibition can be prevented by an E2 antagonist or P4. This E2 inhibition may occur indirectly via the cumulus cells and inhibition of P4 synthesis.</description><identifier>ISSN: 0916-8818</identifier><identifier>EISSN: 1348-4400</identifier><identifier>DOI: 10.1262/jrd.50.305</identifier><identifier>PMID: 15226595</identifier><language>eng</language><publisher>Japan</publisher><subject>Animals ; Cell Culture Techniques - methods ; Cell Nucleus - metabolism ; CULTURE MEDIA ; Culture Media - chemistry ; Estradiol - analogs &amp; derivatives ; Estradiol - pharmacology ; Estrogen Antagonists - pharmacology ; Estrogens - metabolism ; Female ; Fulvestrant ; IN VITRO EXPERIMENTATION ; IN VITRO FERTILIZATION ; Male ; MATURATION ; OESTROGENS ; Oocytes - drug effects ; Oocytes - metabolism ; OVA ; Progesterone - metabolism ; Spermatozoa - metabolism ; SWINE</subject><ispartof>The Journal of reproduction and development, 2004-06, Vol.50 (3), p.305-313</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2455-4ad24ab8ba56874386de0e11b08a593dca059e17b32c964aa0e9ec49f518310e3</citedby><cites>FETCH-LOGICAL-c2455-4ad24ab8ba56874386de0e11b08a593dca059e17b32c964aa0e9ec49f518310e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,864,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15226595$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Q. (Okayama Univ. (Japan))</creatorcontrib><creatorcontrib>Niwa, K</creatorcontrib><creatorcontrib>Hunter, M.G</creatorcontrib><title>Effects of 17beta-estradiol on in vitro maturation of pig oocytes in protein-free medium</title><title>The Journal of reproduction and development</title><addtitle>J Reprod Dev</addtitle><description>The present study examined the effects of 17beta-estradiol (E2) on in vitro maturation and subsequent in vitro fertilization of pig oocytes matured with or without cumulus cells. When E2 (10 ng/ml) was added to the protein-free maturation medium, the proportions of cumulus-enclosed oocytes that underwent germinal vesicle breakdown and reached metaphase II were significantly reduced (P&lt;0.05), and cumulus expansion was also significantly inhibited (P&lt;0.05) compared with the control (no E2 added). Although oocytes matured in the presence of E2 were penetrated by sperm in vitro at the same level as the control, the incidences of male pronuclear (MPN) formation and activated oocytes were significantly lower (P&lt;0.05) than the control. These inhibitory effects of E2 were prevented when the medium was supplemented with E2 together with its antagonist, ICI 182,780 (1 micro g/ml), although the presence of the antagonist alone in the medium had no effect on the maturation and fertilization in vitro of oocytes. In cumulus-free oocytes, E2 had no effect on nuclear maturation and penetration in vitro, but low MPN formation was observed in oocytes matured in the presence and absence of E2. When cumulus-enclosed oocytes were cultured in the presence of progesterone (P4; 600 ng/ml) alone or together with E2, no significant differences in nuclear maturation, cumulus expansion or penetration in vitro were observed compared with control oocytes. The concentration of P4 in maturation medium was significantly (P&lt;0.01) lower when cumulus-enclosed oocytes were cultured for 44 h in the medium with E2 than in medium without E2. These results indicate that E2 inhibits both nuclear and cytoplasmic maturation of cumulus-enclosed pig oocytes, and that this inhibition can be prevented by an E2 antagonist or P4. This E2 inhibition may occur indirectly via the cumulus cells and inhibition of P4 synthesis.</description><subject>Animals</subject><subject>Cell Culture Techniques - methods</subject><subject>Cell Nucleus - metabolism</subject><subject>CULTURE MEDIA</subject><subject>Culture Media - chemistry</subject><subject>Estradiol - analogs &amp; derivatives</subject><subject>Estradiol - pharmacology</subject><subject>Estrogen Antagonists - pharmacology</subject><subject>Estrogens - metabolism</subject><subject>Female</subject><subject>Fulvestrant</subject><subject>IN VITRO EXPERIMENTATION</subject><subject>IN VITRO FERTILIZATION</subject><subject>Male</subject><subject>MATURATION</subject><subject>OESTROGENS</subject><subject>Oocytes - drug effects</subject><subject>Oocytes - metabolism</subject><subject>OVA</subject><subject>Progesterone - metabolism</subject><subject>Spermatozoa - metabolism</subject><subject>SWINE</subject><issn>0916-8818</issn><issn>1348-4400</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNpF0M9LwzAUB_AgipvTi3elJw9C58uvNj3KmL8Y6EHBW0jb15HRNjNphf33ZmxgLg_Ch8f3fQm5pjCnLGMPG1_PJcw5yBMypVyoVAiAUzKFgmapUlRNyEUIGwDOZCbOyYRKxjJZyCn5XjYNVkNIXJPQvMTBpBgGb2rr2sT1ie2TXzt4l3RmGL0ZbPyLdGvXiXPVbsCwJ1vvBrR92njEpMPajt0lOWtMG_DqOGfk62n5uXhJV-_Pr4vHVVoxIWUqTM2EKVVpZKZywVVWIyClJSgjC15XBmSBMRlnVZEJYwALrETRSKo4BeQzcnfYGzP8jDG77myosG1Nj24MOosvB8gjvD_AyrsQPDZ6621n_E5T0PsedexRS9Cxx4hvj1vHMt7zT4_FRXBzAI1x2qy9DfrtgwFIABoB_wOaMnZs</recordid><startdate>200406</startdate><enddate>200406</enddate><creator>Li, Q. (Okayama Univ. (Japan))</creator><creator>Niwa, K</creator><creator>Hunter, M.G</creator><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200406</creationdate><title>Effects of 17beta-estradiol on in vitro maturation of pig oocytes in protein-free medium</title><author>Li, Q. (Okayama Univ. (Japan)) ; Niwa, K ; Hunter, M.G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2455-4ad24ab8ba56874386de0e11b08a593dca059e17b32c964aa0e9ec49f518310e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Cell Culture Techniques - methods</topic><topic>Cell Nucleus - metabolism</topic><topic>CULTURE MEDIA</topic><topic>Culture Media - chemistry</topic><topic>Estradiol - analogs &amp; derivatives</topic><topic>Estradiol - pharmacology</topic><topic>Estrogen Antagonists - pharmacology</topic><topic>Estrogens - metabolism</topic><topic>Female</topic><topic>Fulvestrant</topic><topic>IN VITRO EXPERIMENTATION</topic><topic>IN VITRO FERTILIZATION</topic><topic>Male</topic><topic>MATURATION</topic><topic>OESTROGENS</topic><topic>Oocytes - drug effects</topic><topic>Oocytes - metabolism</topic><topic>OVA</topic><topic>Progesterone - metabolism</topic><topic>Spermatozoa - metabolism</topic><topic>SWINE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Q. (Okayama Univ. (Japan))</creatorcontrib><creatorcontrib>Niwa, K</creatorcontrib><creatorcontrib>Hunter, M.G</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of reproduction and development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Q. (Okayama Univ. (Japan))</au><au>Niwa, K</au><au>Hunter, M.G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of 17beta-estradiol on in vitro maturation of pig oocytes in protein-free medium</atitle><jtitle>The Journal of reproduction and development</jtitle><addtitle>J Reprod Dev</addtitle><date>2004-06</date><risdate>2004</risdate><volume>50</volume><issue>3</issue><spage>305</spage><epage>313</epage><pages>305-313</pages><issn>0916-8818</issn><eissn>1348-4400</eissn><abstract>The present study examined the effects of 17beta-estradiol (E2) on in vitro maturation and subsequent in vitro fertilization of pig oocytes matured with or without cumulus cells. When E2 (10 ng/ml) was added to the protein-free maturation medium, the proportions of cumulus-enclosed oocytes that underwent germinal vesicle breakdown and reached metaphase II were significantly reduced (P&lt;0.05), and cumulus expansion was also significantly inhibited (P&lt;0.05) compared with the control (no E2 added). Although oocytes matured in the presence of E2 were penetrated by sperm in vitro at the same level as the control, the incidences of male pronuclear (MPN) formation and activated oocytes were significantly lower (P&lt;0.05) than the control. These inhibitory effects of E2 were prevented when the medium was supplemented with E2 together with its antagonist, ICI 182,780 (1 micro g/ml), although the presence of the antagonist alone in the medium had no effect on the maturation and fertilization in vitro of oocytes. In cumulus-free oocytes, E2 had no effect on nuclear maturation and penetration in vitro, but low MPN formation was observed in oocytes matured in the presence and absence of E2. When cumulus-enclosed oocytes were cultured in the presence of progesterone (P4; 600 ng/ml) alone or together with E2, no significant differences in nuclear maturation, cumulus expansion or penetration in vitro were observed compared with control oocytes. The concentration of P4 in maturation medium was significantly (P&lt;0.01) lower when cumulus-enclosed oocytes were cultured for 44 h in the medium with E2 than in medium without E2. These results indicate that E2 inhibits both nuclear and cytoplasmic maturation of cumulus-enclosed pig oocytes, and that this inhibition can be prevented by an E2 antagonist or P4. This E2 inhibition may occur indirectly via the cumulus cells and inhibition of P4 synthesis.</abstract><cop>Japan</cop><pmid>15226595</pmid><doi>10.1262/jrd.50.305</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0916-8818
ispartof The Journal of reproduction and development, 2004-06, Vol.50 (3), p.305-313
issn 0916-8818
1348-4400
language eng
recordid cdi_proquest_miscellaneous_66667007
source Directory of Open Access Journals
subjects Animals
Cell Culture Techniques - methods
Cell Nucleus - metabolism
CULTURE MEDIA
Culture Media - chemistry
Estradiol - analogs & derivatives
Estradiol - pharmacology
Estrogen Antagonists - pharmacology
Estrogens - metabolism
Female
Fulvestrant
IN VITRO EXPERIMENTATION
IN VITRO FERTILIZATION
Male
MATURATION
OESTROGENS
Oocytes - drug effects
Oocytes - metabolism
OVA
Progesterone - metabolism
Spermatozoa - metabolism
SWINE
title Effects of 17beta-estradiol on in vitro maturation of pig oocytes in protein-free medium
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-07T15%3A59%3A21IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effects%20of%2017beta-estradiol%20on%20in%20vitro%20maturation%20of%20pig%20oocytes%20in%20protein-free%20medium&rft.jtitle=The%20Journal%20of%20reproduction%20and%20development&rft.au=Li,%20Q.%20(Okayama%20Univ.%20(Japan))&rft.date=2004-06&rft.volume=50&rft.issue=3&rft.spage=305&rft.epage=313&rft.pages=305-313&rft.issn=0916-8818&rft.eissn=1348-4400&rft_id=info:doi/10.1262/jrd.50.305&rft_dat=%3Cproquest_cross%3E66667007%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c2455-4ad24ab8ba56874386de0e11b08a593dca059e17b32c964aa0e9ec49f518310e3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=66667007&rft_id=info:pmid/15226595&rfr_iscdi=true