Serological diagnosis of tuberculosis

Objective To evaluate the efficacy of ELISA for the detection of IgG antibodies against antigen 85 complex (Ag 85 complex) of Mycobacterium tuberculosis . Methods Children of either sex, 0-18 years of age, attending the outpatient department and admitted in the casualty and wards of the Department o...

Full description

Saved in:
Bibliographic Details
Published in:Indian journal of pediatrics 2008-12, Vol.75 (12), p.1219-1221
Main Authors: Dayal, R., Singh, A., Katoch, V. M., Joshi, B., Chauhan, D.S., Singh, P., Kumar, G., Sharma, V.D.
Format: Article
Language:English
Subjects:
Acyltransferases - immunology
Adolescent
Antigens, Bacterial - immunology
Bacterial diseases
Bacteriological Techniques
Biological and medical sciences
Child
Child, Preschool
Enzyme-Linked Immunosorbent Assay
Female
General aspects
Gynecology
Human bacterial diseases
Humans
Immunoglobulin G - blood
Infant
Infant, Newborn
Infectious diseases
Male
Medical sciences
Medicine
Medicine & Public Health
Original Article
Pediatrics
Sensitivity and Specificity
Serologic Tests - methods
Tuberculosis - diagnosis
Tuberculosis and atypical mycobacterial infections
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Objective To evaluate the efficacy of ELISA for the detection of IgG antibodies against antigen 85 complex (Ag 85 complex) of Mycobacterium tuberculosis . Methods Children of either sex, 0-18 years of age, attending the outpatient department and admitted in the casualty and wards of the Department of Pediatrics, S.N. Medical College, Agra, were included in present study. The study was carried out on children with pulmonary and CNS tuberculosis along with matching controls (83 cases and 32 controls). Informed consents of their parents or guardians were taken. They were subjected to clinical examination, relevant laboratory investigations, tuberculin test and chest radiograph. Relevant body fluids were subjected to bacteriological tests; ELISA was applied to serum samples for detection of IgG antibodies against antigen 85 complex (Ag85). The result of ELISA was compared with bacteriological tests [Ziehl Neelson (ZN) staining for acid-fast bacilli, culture on Lowenstein Jensen (LJ) medium and culture on BacT/Alert 3D system]. Results ELISA tests showed a significantly higher sensitivity (59.1%) as compared with LJ medium culture method (19.3%), BacT/Alert 3D system (24.1%) and ZN staining (16.9%) in all patients (p
ISSN:0019-5456
0973-7693
DOI:10.1007/s12098-008-0222-3