Neural Activity Triggers Neuronal Oxidative Metabolism Followed by Astrocytic Glycolysis

We have found that two-photon fluorescence imaging of nicotinamide adenine dinucleotide (NADH) provides the sensitivity and spatial three-dimensional resolution to resolve metabolic signatures in processes of astrocytes and neurons deep in highly scattering brain tissue slices. This functional imagi...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2004-07, Vol.305 (5680), p.99-103
Main Authors: Kasischke, Karl A., Vishwasrao, Harshad D., Fisher, Patricia J., Zipfel, Warren R., Webb, Watt W.
Format: Article
Language:English
Subjects:
Animals
Astrocytes
Astrocytes - metabolism
Behavioral neuroscience
Biological and medical sciences
Brain
Brain chemistry
Citric Acid Cycle
Cytoplasm
Dendrites
Dendrites - metabolism
Electron Transport
Energy metabolism
Fluorescence
Fundamental and applied biological sciences. Psychology
Glycolysis
Hippocampus - cytology
Hippocampus - metabolism
In Vitro Techniques
Lactates
Lactic Acid - metabolism
Medical imaging
Metabolism
Mitochondria
Mitochondria - metabolism
NAD - metabolism
Neurology
Neurons
Neurons - metabolism
Neuropil
Oxidation-Reduction
Oxygen Consumption
Pyramidal Cells - metabolism
Rats
Rats, Sprague-Dawley
Spectrometry, Fluorescence
Vertebrates: nervous system and sense organs
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Summary:We have found that two-photon fluorescence imaging of nicotinamide adenine dinucleotide (NADH) provides the sensitivity and spatial three-dimensional resolution to resolve metabolic signatures in processes of astrocytes and neurons deep in highly scattering brain tissue slices. This functional imaging reveals spatiotemporal partitioning of glycolytic and oxidative metabolism between astrocytes and neurons during focal neural activity that establishes a unifying hypothesis for neurometabolic coupling in which early oxidative metabolism in neurons is eventually sustained by late activation of the astrocyte-neuron lactate shuttle. Our model integrates existing views of brain energy metabolism and is in accord with known macroscopic physiological changes in vivo.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.1096485