Osteopontin Expression Profiles Predict Pathological and Clinical Outcome in Breast Cancer

Background: Osteopontin (OPN) overexpression in breast cancer has been associated with adverse pathological and clinical outcomes. In this study, the OPN expression profiles were examined in a cohort of breast cancer patients. Patients and Methods: RNA extraction and reverse transcription were perfo...

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Bibliographic Details
Published in:Anticancer research 2008-11, Vol.28 (6B), p.4105-4110
Main Authors: PATANI, Neill, JOUHRA, Fadi, WEN JIANG, MOKBEL, Kefah
Format: Article
Language:English
Subjects:
Biological and medical sciences
Breast Neoplasms - genetics
Breast Neoplasms - metabolism
Breast Neoplasms - pathology
Disease-Free Survival
Female
Gynecology. Andrology. Obstetrics
Humans
Mammary gland diseases
Medical sciences
Osteopontin - biosynthesis
Osteopontin - genetics
Protein Isoforms
RNA, Messenger - biosynthesis
RNA, Messenger - genetics
Survival Rate
Tumors
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Summary:Background: Osteopontin (OPN) overexpression in breast cancer has been associated with adverse pathological and clinical outcomes. In this study, the OPN expression profiles were examined in a cohort of breast cancer patients. Patients and Methods: RNA extraction and reverse transcription were performed on breast carcinomas (n=127) and normal tissues (n=33). Transcript levels were determined using real-time PCR. Results: The OPN-a levels decreased with increasing TNM stage and worse clinical outcome. The OPN-b levels increased with tumour grade and Nottingham Prognostic Index (NPI) stage, were higher in patients who died of breast cancer than in those who were disease-free after 10 years and predicted disease-free survival. The OPN-c expression was associated with tumour grade and poor prognosis. Furthermore, the expression levels predicted local recurrence, disease-free survival and bone metastases. Conclusion: OPN expression profiles are significantly associated with tumour grade, stage and patient prognosis in breast cancer. OPN-c is likely to be of particular utility as a prognostic marker and should be included in future validation studies.
ISSN:0250-7005
1791-7530