Naturally Acquired IgM Antibody Response to the C-Terminal Region of the Merozoite Surface Protein 1 of Plasmodium vivax in Korea: Use for Serodiagnosis of Vivax Malaria

• Published in: The Journal of parasitology 2008-12, Vol.94 (6), p.1410-1414
• Main Authors: Yeom, Joon-Sup, Kim, Eu-Suk, Lim, Kook-Jin, Oh, Jae-Hoon, Sohn, Mi-Jin, Yoo, Seung-Bum, Kim, Eunkyung, Bae, Ineui, Jung, Yun-Jae, Park, Jae-Won
• Format: Article
• Language: English
• Subjects: Animals; Antibodies; Antibodies, Protozoan - biosynthesis; Antibodies, Protozoan - blood; Antibody formation; Biological and medical sciences; Blood; Blood & organ donations; Case-Control Studies; Control groups; E coli; Enzyme linked immunosorbent assay; Enzyme-Linked Immunosorbent Assay - methods; Enzyme-Linked Immunosorbent Assay - standards; Follow-Up Studies; Fundamental and applied biological sciences. Psychology; General aspects; General aspects and techniques. Study of several systematic groups. Models; Humans; Immunoglobulin M - biosynthesis; Immunoglobulin M - blood; Immunoglobulins; Infections; Invertebrates; Korea; Malaria; Malaria, Vivax - diagnosis; Mass Screening - methods; Merozoite Surface Protein 1 - immunology; Merozoites; Patients; Plasmodium vivax; Plasmodium vivax - immunology; Proteins; Recurrence; Sandwiches; Sensitivity and Specificity; Therapeutics-Diagnostics; Vector-borne diseases; Vivax malaria
• ISSN: 0022-3395; 1937-2345
• DOI: 10.1645/GE-1484.1

The antibody levels against the C-terminal region of the merozoite surface protein 1 of Plasmodium vivax (PvMSP1c) were measured in 276 patients with P. vivax malaria (patient group), 320 malaria-naïve healthy individuals (control group 1), and 70 malaria-naïve individuals with various disorders (control group 2) using the immunoglobulin M (IgM) capture enzyme-linked immunosorbent assay (ELISA) and the direct sandwich ELISA. To evaluate the antibody response during relapse, 5 relapsed patients were tested using the IgM capture ELISA. The IgM antibodies were negative in 99.7% of control group 1 and in 100% of control group 2; they were positive in 90.6% of the patient group. The total antibody levels were positive in 88.4% of the patient group with the direct sandwich ELISA. The sera from the second malaria episode, i.e., relapsed patients, were 100% positive on the IgM capture ELISA. The results of this study suggest that the IgM capture ELISA may be a useful diagnostic method for P. vivax malaria for both primary infection and relapse.