A circular dichroism study of the stability of guanine quadruplexes of thrombin DNA aptamers at presence of K+ and Na+ ions

The effect of K+ and Na+ on the properties of DNA aptamers that selective binds to fibrinogen (FIBRI) and heparin (HEPA) exosites of human alpha-thrombin was studied by circular dichroism (CD). The complexes of FIBRI-K+ were slightly more stable than HEPA-K+. However, lower stability was observed fo...

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Published in:General physiology and biophysics 2008-12, Vol.27 (4), p.271-277
Main Authors: Poniková, Slavomíra, Antalík, Marián, Hianik, Tibor
Format: Article
Language:English
Subjects:
Aptamers, Nucleotide - chemistry
Cations - chemistry
Circular Dichroism - methods
Fibrinogen - chemistry
G-Quadruplexes
Heparin - chemistry
Humans
Potassium - chemistry
Sodium - chemistry
Thrombin - chemistry
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creator Poniková, Slavomíra
Antalík, Marián
Hianik, Tibor
description The effect of K+ and Na+ on the properties of DNA aptamers that selective binds to fibrinogen (FIBRI) and heparin (HEPA) exosites of human alpha-thrombin was studied by circular dichroism (CD). The complexes of FIBRI-K+ were slightly more stable than HEPA-K+. However, lower stability was observed for HEPA-K+ at presence of Na+ in comparison with FIBRI-K+. The analysis of CD melting curves suggests differences in thermal stability of both aptamers at presence of K+. The melting temperatures (Tm) and changes in van't Hoff enthalpy for HEPA-K+ complexes were lower in comparison with those for FIBRI-K+. With increasing HEPA concentration the Tm value increased, but Tm did not change with increasing FIBRI concentration. This suggests formation of HEPA aggregates, while FIBRI aptamers are in monomeric form.
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subjects Aptamers, Nucleotide - chemistry
Cations - chemistry
Circular Dichroism - methods
Fibrinogen - chemistry
G-Quadruplexes
Heparin - chemistry
Humans
Potassium - chemistry
Sodium - chemistry
Thrombin - chemistry
title A circular dichroism study of the stability of guanine quadruplexes of thrombin DNA aptamers at presence of K+ and Na+ ions
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