Simultaneous Determination of Some Phenothiazine Derivatives in Human Blood by Headspace Solid-Phase Microextraction and Gas Chromatography with Nitrogen-Phosphorus Detection

Chlorpromazine, levomepromazine, promazine, triflupromazine, and trimeprazine were simultaneously determined in human whole blood and plasma by combining headspace solid-phase microextraction and gas chromatography with nitrogen-phosphorus detection. Extraction efficiency for the phenothiazine deriv...

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Bibliographic Details
Published in:Journal of AOAC International 2008-11, Vol.91 (6), p.1354-1362
Main Authors: SHINMEN, Natsuko, LEE, Xiao-Pen, KUMAZAWA, Takeshi, HASEGAWA, Chika, ISHIWATA, Yasuhiro, SATO, Keizo, SENO, Hiroshi, SUZUKI, Osamu
Format: Article
Language:English
Subjects:
Adult
Antipsychotic Agents - blood
Biological and medical sciences
Blood plasma
Chromatography, Gas
Composition
Extraction (Chemistry)
Female
Food industries
Fundamental and applied biological sciences. Psychology
Gas chromatography
General aspects
Humans
Identification and classification
Indicators and Reagents
Methods
Methods of analysis, processing and quality control, regulation, standards
Nitrogen - chemistry
Nitrogen compounds
Phenothiazine
Phenothiazines - blood
Phosphorus - chemistry
Properties
Quality Control
Reference Standards
Solid Phase Microextraction
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Summary:Chlorpromazine, levomepromazine, promazine, triflupromazine, and trimeprazine were simultaneously determined in human whole blood and plasma by combining headspace solid-phase microextraction and gas chromatography with nitrogen-phosphorus detection. Extraction efficiency for the phenothiazine derivatives was 0.013-0.117% for both sample types. Regression equations were linear [correlation coefficient (r) = 0.9951-0.9999] within the range 2.5-200 ng/0.5 mL for triflupromazine and trimeprazine, and 6.3-200 ng/0.5 mL for chlorpromazine, levomepromazine, and promazine. The limit of detection for each compound was 0.2-3.9 ng/0.5 mL whole blood and plasma. Intraday and interday coefficients of variation for all phenothiazines in both human samples were commonly < 15 and 20%, respectively. We also report the determination of levomepromazine in human plasma after oral administration.
ISSN:1060-3271
1944-7922
DOI:10.1093/jaoac/91.6.1354