Synergistic activity of Paenibacillus sp. BP-23 cellobiohydrolase Cel48C in association with the contiguous endoglucanase Cel9B and with endo- or exo-acting glucanases from Thermobifida fusca

Cellobiohydrolase Cel48C from Paenibacillus sp. BP-23, an enzyme displaying limited activity on most cellulosic substrates, was assayed for activity in the presence of other bacterial endo- or exocellulases. Significant enhanced activity was observed when Cel48C was incubated in the presence of Paen...

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Bibliographic Details
Published in:Biotechnology and bioengineering 2004-07, Vol.87 (2), p.161-169
Main Authors: Sanchez, M.M, Irwin, D.C, Pastor, F.I.J, Wilson, D.B, Diaz, P
Format: Article
Language:English
Subjects:
Actinomycetales - enzymology
Bacillus - enzymology
Bacillus - growth & development
Bacteria
Biological and medical sciences
Biotechnology
Carboxymethylcellulose Sodium - metabolism
cellobiohydrolase
cellobiose
Cellobiose - analysis
Cellobiose - metabolism
cellulase
Cellulase - chemistry
Cellulase - genetics
Cellulase - metabolism
cellulases
Cellulose - metabolism
Cellulose 1,4-beta-Cellobiosidase - chemistry
Cellulose 1,4-beta-Cellobiosidase - genetics
Cellulose 1,4-beta-Cellobiosidase - metabolism
cellulose degradation
Chromatography, Thin Layer
Electrophoresis, Polyacrylamide Gel
Enzymes
Escherichia coli
Escherichia coli - genetics
Fundamental and applied biological sciences. Psychology
Glucose - metabolism
Glycoside Hydrolases - chemistry
Glycoside Hydrolases - genetics
Glycoside Hydrolases - metabolism
Isoelectric Point
Kinetics
Oligosaccharides - analysis
Paenibacillus
Recombinant Proteins - metabolism
Substrate Specificity
synergism
Thermobifida fusca
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Summary:Cellobiohydrolase Cel48C from Paenibacillus sp. BP-23, an enzyme displaying limited activity on most cellulosic substrates, was assayed for activity in the presence of other bacterial endo- or exocellulases. Significant enhanced activity was observed when Cel48C was incubated in the presence of Paenibacillus sp. BP-23 endoglucanase Cel9B or Thermobifida fusca cellulases Cel6A and Cel6B, indicating that Cel48C acts synergistically with them. Maximum synergism rates on bacterial microcrystalline cellulose or filter paper were obtained with a mixture of Paenibacillus cellulases Cel9B and Cel48C, accompanied by T. fusca exocellulase Cel6B. Synergism was also observed in cell extracts from recombinant clone E. coli pUCel9-Cel48 expressing the two contiguous Paenibacillus cellulases Cel9B and Cel48C. The enhanced cellulolytic activity displayed by the cellulase mixtures assayed could be used as an efficient tool for biotechnological applications like pulp and paper manufacturing.
ISSN:0006-3592
1097-0290
DOI:10.1002/bit.20099