A Four-Color Colloidal Multiplexing Nanoparticle System

Any labeling with multicolor markers can be affected by the autofluorescence of biological tissue due to the UV or blue light excitation sources, or the results are affected by fluorescence resonance energy transfer. In this work, we present novel IR-to-vis upconverting nanoparticles of different ra...

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Bibliographic Details
Published in:ACS nano 2008-01, Vol.2 (1), p.120-124
Main Authors: Ehlert, Oliver, Thomann, Ralf, Darbandi, Masih, Nann, Thomas
Format: Article
Language:English
Subjects:
Colloids
Color
Lanthanoid Series Elements - chemistry
Luminescence
Microscopy, Electron, Transmission
Nanoparticles - chemistry
Spectrophotometry, Ultraviolet
X-Ray Diffraction
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Summary:Any labeling with multicolor markers can be affected by the autofluorescence of biological tissue due to the UV or blue light excitation sources, or the results are affected by fluorescence resonance energy transfer. In this work, we present novel IR-to-vis upconverting nanoparticles of different rare earth metal dopants. With a single excitation source of 980 nm, four different colors of nanocrystals can be spectrally separated under multiplexing conditions. The particles were phase transferred into polar solvents by means of silica encapsulation and were characterized by transmission electron microscopy, X-ray diffraction, and photoluminescence spectroscopy.
ISSN:1936-0851
1936-086X
DOI:10.1021/nn7002458