Mast cell mediator release in nonasthmatic subjects after endobronchial adenosine challenge

Adenosine 5′-monophosphate (AMP) has been shown to cause bronchoconstriction in atopic subjects but to have no effect on nonatopic nonasthmatic subjects. Endobronchial AMP challenge has previously been shown to cause mast cell mediator release in asthmatic subjects, but it is unknown whether a simil...

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Bibliographic Details
Published in:Journal of allergy and clinical immunology 2004-07, Vol.114 (1), p.34-39
Main Authors: Crummy, Fionnuala, Livingston, Mark, Ennis, Madeleine, Heaney, Liam G
Format: Article
Language:English
Subjects:
adenosine
Adenosine Monophosphate - pharmacology
Adolescent
Adult
Asthma
Asthma - immunology
Biological and medical sciences
Bronchial Provocation Tests - methods
Bronchoconstriction - drug effects
Bronchoconstriction - immunology
Bronchoconstrictor Agents - pharmacology
bronchoscopy
endobronchial challenge
Female
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Humans
Hypersensitivity - immunology
Immunopathology
Inflammation Mediators - immunology
Male
Mast cell
Mast Cells - immunology
Medical sciences
Proteins
Skin
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Summary:Adenosine 5′-monophosphate (AMP) has been shown to cause bronchoconstriction in atopic subjects but to have no effect on nonatopic nonasthmatic subjects. Endobronchial AMP challenge has previously been shown to cause mast cell mediator release in asthmatic subjects, but it is unknown whether a similar response occurs in atopic nonasthmatic and nonatopic nonasthmatic control subjects who have no response to inhalation AMP challenge. This study examined the change in mast cell–derived products after endobronchial saline challenge and AMP challenge in subjects with and without a positive inhalation response to AMP. Inhalation challenge with AMP challenge was performed in normal, atopic nonasthmatic, and atopic asthmatic subjects. Levels of mast cell mediators were measured after endobronchial adenosine challenge and after placebo endobronchial saline challenge. There were significant increases in histamine, tryptase, protein, and prostaglandin D2 levels (P=.02, P=.02, P=.01, and P=.01, respectively) after AMP challenge compared with after saline challenge in nonatopic nonasthmatic subjects. There was no significant increase in any mediator in either of the other 2 groups. This study suggests dissociation between mediator release and bronchoconstriction in response to AMP.
ISSN:0091-6749
1097-6825
DOI:10.1016/j.jaci.2004.03.006